Transcriptional Response of the Murine Mammary Gland to Acute Progesterone Exposure
Endocrinology Fernandez-Valdivia et al. 149: 6236 Supplemental Data
Supplemental Data
Files in this Data Supplement:
- Supplemental Figure 1 - Progesterone exposure induces branching morphogenesis in the mammary gland of the ovariectomized mouse
A-F show low magnification images of inguinal (#4) mammary gland whole-mounts from ovariectomized mice that were either untreated, progesterone-treated for 76h, sesame oil-treated for 76h, progesterone-treated for 1wk, progesterone-treated for 2wks, or sesame oil-treated for 2wks respectively. Progesterone-induced ductal side-branching clearly increases with hormone exposure time (compare B, D, and E with A, C, and F). As expected, branching morphogenesis does not occur with sesame oil treatment (compare C and F with A). Scale bar in A applies to panels B-F; the lymph node, which acts as a spatial reference point, is denoted by LN. - Supplemental Figure 2 - Acute progesterone exposure induces mammary epithelial proliferation in the mammary gland of the ovariectomized mouse
A and B show H&E stained sections of mammary tissue obtained from ovariectomized 8 week-old mice treated for 76h with sesame oil and progesterone respectively. Note the presence of numerous mitotic figures present in B (arrowheads). C and D are low and high magnification images of alveolar buds observed in the mammary gland of an 8 week-old ovariectomized mouse treated with progesterone for 2 weeks respectively. Note the persistent presence of mitotic figures in D (arrowhead). Scale bars in A and C apply to B and D respectively. - Supplemental Table 1 - Tabulated display of the total number of Balb/c mice used in the microarray study
Three mouse sets (or separate groups) of mice (with 5 mice within each set or group) were used for each time-point and hormone treatment. A total of 120 mice were used in this microarray study. - Supplemental Table 3 - Table of murine mammary genes induced by both progesterone and prolactin administration
- Supplemental Table 2 - Table of genes up or down-regulated by 4, 16, 28, and 76 hours of progesterone exposure