Files in this Data Supplement:
Fig. S1. Expression of soxE genes in the WT ear diverges during otic vesicle formation. Ears of WT embryos at the 15-somite stage are shown in whole-mount (A-C) and in transverse sections through the anterior (A’-C’), medial (A’ ’-C’ ’) and posterior (A’ ’ ’-C’ ’ ’) regions. In this figure, and all subsequent figures, developing otic structures are shown in a lateral view with anterior to the left and dorsal to the top unless stated otherwise. The apparent cavity (e.g. in A’ ’, B’ ’) is likely to result from shrinkage during processing. Bars, 150 µm (A-C); 50 µm (A’-C’ ’ ’).
Fig. S2. SoxE gene expression in cls mutant embryos at 48 hpf. The ears of cls/sox10 mutants (B,D,F) and their WT siblings (A,C,E) are shown in a lateral view of whole-mount embryos (A-F) and in transverse sections through the anterior (A’-F’), medial (A’’-F’’) and posterior (A’’’-F’’’) regions. At this stage, sox10 expression remains in most or all cells of the WT otic epithelium and is largely unchanged in the ears of cls/sox10 mutants (A-B’’’). Expression of sox9a is downregulated in the WT ear by 48 hpf, but is present in the surrounding mesenchyme (arrowhead, C-C’’’). However, sox9a expression remains strong in the lateral epithelium in cls/sox10 mutants (arrow, D-D’’’). sox9b is strongly expressed at the tips of epithelial projections that form the semicircular canal system (E’’), but is downregulated elsewhere in the vesicle (E-E’’’). Expression of sox9b persists in the dorsal and lateral epithelium of cls/sox10 mutants (arrow), but development of epithelial projections is delayed in the mutant and so these domains of expression are not evident (compare E’’ and F’’). Bars, 75 µm (A-F); 50 µm (A’-F’’’).
