Blood -- ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function

Blood, Vol. 113, Issue 10, 2181-2190, March 5, 2009

ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function
Blood Collins et al. 113: 2181

Supplemental materials for: Collins et al

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Figure S1. FANCA is phosphorylated in vivo on serine 1449 (JPG, 40.8 KB) -
Whole cell lysates from GM6914 (FA-A) fibroblasts expressing Flag-tagged FANCA (lanes 2–5) or vector control (lane 1) treated with 1mM MMC for 18 hours (lanes 4 and 5) and/or 1mM okadaic acid for 30 minutes (lanes 3 and 5) were immunoprecipitated using anti-FLAG antibody, run on SDS-PAGE and silver stained. The band from lane 5 was excised and analyzed by mass spectrometry. IP: immunoprecipitation.
Figure S2. FANCA S1449A localizes to chromatin (JPG, 46 KB) -
GM6914 (FA-A) fibroblasts expressing vector only, Flag-tagged wild type FANCA, or FANCA S1449A mutant were treated or not with 1mM MMC for 18 hours. Chromatin extracts were prepared and run on SDS-PAGE and progressively immunoblotted with phosphospecific antibody and anti-FANCA antibody. Both wild type and mutant FANCA localized to chromatin.