=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= miRanda v1.0b microRNA Target Scanning Algorithm =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= (c) 2003 Memorial Sloan-Kettering Cancer Center, New York Authors: Anton Enright, Bino John, Chris Sander and Debora Marks (mirnatargets@cbio.mskcc.org - reaches all authors) Software written by: Anton Enright Distributed for anyone to use under the GNU Public License (GPL), See the files 'COPYING' and 'LICENSE' for details If you use this software please cite: Enright AJ, John B, Gaul U, Tuschl T, Sander C and Marks DS; (2003) Genome Biology; 5(1):R1. miRanda comes with ABSOLUTELY NO WARRANTY; This is free software, and you are welcome to redistribute it under certain conditions; type `miranda --license' for details. Current Settings: =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Query Filename: d:\docs\Zak\Tools\miRanda\Post Zwi\TAR 3p.txt Reference Filename: d:\docs\Zak\Tools\miRanda\Post Zwi\Experiment new for nature.txt Gap Open Penalty: -2.000000 Gap Extend: -8.000000 Score Threshold 80.000000 Energy Threshold -14.000000 kcal/mol Z-Score Threshold 5.000000 Shuffling Turned on: Shuffles: 150 Window Size: 20 Scaling Parameter: 2.000000 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Read Sequence:TAR 5p (17 nt) Read Sequence:TAR Sequence (21 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 39.65 St. Dev: 6.98 Max: 60.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs TAR =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 111.000000 Q:4 to 18 R:1 to 16 Align Len (15) (93.33%) (93.33%) Query: 3' CACCCA-AGGGAUCAAUC 5' ||| ||||||||||| Ref: 5' †GGUCUCUCUGGUUAG 3' Z-Score: 10.219 Energy: -18.629999 kCal/Mol Scores for this hit: >TAR TAR 111.00 -18.63 10.22 4 18 1 16 15 93.33% 93.33% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR TAR 111.00 -18.63 111.00 -18.63 1 17 21 0 Complete Read Sequence:Human alpha-1 type VII collagen (COL7A1) mRNA, complete cds. (9287 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 63.40 St. Dev: 6.01 Max: 71.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Human =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 101.000000 Q:1 to 18 R:3593 to 3610 Align Len (19) (78.95%) (78.95%) Query: 3' C-ACCCAAGG-GAUCAAUC 5' | | |||||| |||| ||| Ref: 5' GAT-GGTTCTGCTAG-TGG 3' Z-Score: 6.252 Energy: -14.550000 kCal/Mol Scores for this hit: >TAR Human 101.00 -14.55 6.25 1 18 3593 3610 19 78.95% 78.95% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Human 101.00 -14.55 101.00 -14.55 2 17 9287 3592 Complete Read Sequence:Homo sapiens retinal degeneration B beta mRNA, complete cds. (1124 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 55.77 St. Dev: 5.92 Max: 71.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens ARF GTPase-activating protein GIT2 (KIAA0148) mRNA, (2473 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 57.81 St. Dev: 6.06 Max: 72.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens protein inhibitor of activated STAT protein PIASy (1533 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 56.26 St. Dev: 5.97 Max: 72.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:gi|119964722|ref|NM_003897.3| Homo sapiens immediate early response 3 (IER3), mRNA (1254 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 57.76 St. Dev: 6.10 Max: 72.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs gi|119964722|ref|NM_003897.3| =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 95.000000 Q:1 to 18 R:1003 to 1020 Align Len (19) (73.68%) (73.68%) Query: 3' CACCCAAGGGA-UC-AAUC 5' ||||| | | | || |||| Ref: 5' GTGGG-T-CGTAAGTTTAG 3' Z-Score: 6.108 Energy: -14.010000 kCal/Mol Scores for this hit: >TAR gi|119964722|ref|NM_003897.3| 95.00 -14.01 6.11 1 18 1003 1020 19 73.68% 73.68% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR gi|119964722|ref|NM_003897.3| 95.00 -14.01 95.00 -14.01 6 17 1254 1002 Complete Read Sequence:Homo sapiens SBC2 mRNA for sodium bicarbonate cotransporter2, (3238 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 62.14 St. Dev: 6.20 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 104.000000 Q:1 to 18 R:2948 to 2967 Align Len (19) (78.95%) (78.95%) Query: 3' CACC-CAAGGGA-UCAAUC 5' |||| | ||||| | |||| Ref: 5' GTGGTGATCCCTCAATTGG 3' Z-Score: 6.756 Energy: -15.760000 kCal/Mol Scores for this hit: >TAR Homo 104.00 -15.76 6.76 1 18 2948 2967 19 78.95% 78.95% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Homo 104.00 -15.76 104.00 -15.76 7 17 3238 2947 Complete Read Sequence:Human (HepG2) glucose transporter gene mRNA, complete cds. (2856 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 61.23 St. Dev: 6.21 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Human =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 103.000000 Q:1 to 16 R:1463 to 1480 Align Len (18) (77.78%) (77.78%) Query: 3' CAC-CCAAGGGAU-CA-AUC 5' ||| || |||||| || | Ref: 5' GTGTGG-TCCCTACGTCTTC 3' Z-Score: 6.723 Energy: -14.910000 kCal/Mol Scores for this hit: >TAR Human 103.00 -14.91 6.72 1 16 1463 1480 18 77.78% 77.78% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Human 103.00 -14.91 103.00 -14.91 8 17 2856 1462 Complete Read Sequence:Homo sapiens mRNA for 39k3 protein. (4058 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 60.79 St. Dev: 6.13 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 92.000000 Q:3 to 18 R:1018 to 1035 Align Len (18) (72.22%) (72.22%) Query: 3' CACCC-A-A-GGGAUCAAUC 5' ||| | ||||| |||| Ref: 5' AAGGGATGAACCCTA-TTAG 3' Z-Score: 5.095 Energy: -15.300000 kCal/Mol Scores for this hit: >TAR Homo 92.00 -15.30 5.09 3 18 1018 1035 18 72.22% 72.22% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Homo 92.00 -15.30 92.00 -15.30 9 17 4058 1017 Complete Read Sequence:Homo sapiens TIMM13b mRNA, complete cds. (288 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 50.39 St. Dev: 6.02 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens CGI-21 protein mRNA, complete cds. (2166 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 59.58 St. Dev: 6.00 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 90.000000 Q:1 to 18 R:1788 to 1806 Align Len (18) (77.78%) (77.78%) Query: 3' CACCCAAGGGAU-CAAUC 5' ||||| |||||| | || Ref: 5' GTGGGCTTCCTAGGCCAG 3' Z-Score: 5.071 Energy: -20.740000 kCal/Mol Scores for this hit: >TAR Homo 90.00 -20.74 5.07 1 18 1788 1806 18 77.78% 77.78% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Homo 90.00 -20.74 90.00 -20.74 11 17 2166 1787 Complete Read Sequence:H.sapiens mRNA for RanBP3 (59 kDa). (3198 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 59.76 St. Dev: 6.29 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs H.sapiens =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 97.000000 Q:3 to 18 R:1104 to 1119 Align Len (16) (81.25%) (81.25%) Query: 3' CACCCAAGGGAUC-AAUC 5' ||| ||||||| | || Ref: 5' AAGGG-TCCCTGGCTGAG 3' Z-Score: 5.924 Energy: -19.610001 kCal/Mol Scores for this hit: >TAR H.sapiens 97.00 -19.61 5.92 3 18 1104 1119 16 81.25% 81.25% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR H.sapiens 97.00 -19.61 97.00 -19.61 12 17 3198 1103 Complete Read Sequence:Homo sapiens mRNA for ferrochelatase, complete cds. (2443 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 60.06 St. Dev: 6.32 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 100.000000 Q:1 to 18 R:946 to 964 Align Len (18) (77.78%) (77.78%) Query: 3' CACCCAA-GGGAUCAAUC 5' || | | |||||||||| Ref: 5' GTCCGATGCCCTGGTTGG 3' Z-Score: 6.320 Energy: -17.480000 kCal/Mol Scores for this hit: >TAR Homo 100.00 -17.48 6.32 1 18 946 964 18 77.78% 77.78% Forward: Score: 92.000000 Q:1 to 18 R:1311 to 1330 Align Len (20) (70.00%) (70.00%) Query: 3' CACCCAAGGG-A-U-CAAUC 5' |||| ||| | | ||||| Ref: 5' GTGGAC-CCCGTGGCGTTAG 3' Z-Score: 5.054 Energy: -14.140000 kCal/Mol Scores for this hit: >TAR Homo 92.00 -14.14 5.05 1 18 1311 1330 20 70.00% 70.00% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Homo 192.00 -31.62 100.00 -17.48 13 17 2443 945 1310 Complete Read Sequence:Homo sapiens ubiquinol-cytochrome c reductase binding protein, mRNA (965 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 56.06 St. Dev: 6.05 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens KAL1 gene, VIRTUAL TRANSCRIPT, partial sequence, (1473 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 56.13 St. Dev: 6.15 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens microfibrillar-associated protein 1 (MFAP1), mRNA. (2063 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 59.38 St. Dev: 6.30 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 94.000000 Q:1 to 18 R:233 to 253 Align Len (21) (66.67%) (66.67%) Query: 3' C-A-CCC-A-AGGGAUCAAUC 5' | | ||| |||| |||| | Ref: 5' GCTGGGGCCGTCCC-AGTTCG 3' Z-Score: 5.496 Energy: -16.750000 kCal/Mol Scores for this hit: >TAR Homo 94.00 -16.75 5.50 1 18 233 253 21 66.67% 66.67% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Homo 94.00 -16.75 94.00 -16.75 16 17 2063 232 Complete Read Sequence:Homo sapiens lectin, galactoside-binding, soluble, 1 (galectin 1) (586 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 54.74 St. Dev: 6.27 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens excision repair cross-complementing rodent repair (1273 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 57.34 St. Dev: 6.07 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Score for this Scan: No Hits Found above Threshold Complete Read Sequence:Homo sapiens surfeit 1 (SURF1), nuclear gene encoding mitochondrial (1037 nt) =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Generating Alignment Distribution of Shuffled Sequences =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= done Average: 57.52 St. Dev: 6.42 Max: 74.00 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Performing Scan: TAR vs Homo =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 94.000000 Q:1 to 18 R:916 to 935 Align Len (20) (75.00%) (75.00%) Query: 3' CACCCAAG-GGAUCA-AU-C 5' ||||| | |||||| || | Ref: 5' GTGGGA-CACCTGGTGTGTG 3' Z-Score: 5.686 Energy: -17.360001 kCal/Mol Scores for this hit: >TAR Homo 94.00 -17.36 5.69 1 18 916 935 20 75.00% 75.00% Score for this Scan: Seq1,Seq2,Tot Score,Tot Energy,Max Score,Max Energy,Strand,Len1,Len2,Positions >>TAR Homo 94.00 -17.36 94.00 -17.36 19 17 1037 915 Complete Run Complete