Blood, Vol. 113, Issue 14, 3190-3197, April 2, 2009

Circulating Ly-6C⁺ myeloid precursors migrate to the CNS and play a pathogenic role during autoimmune demyelinating disease
Blood King et al. 113: 3190

Supplemental materials for: King et al

Files in this Data Supplement:

• Figure S1. Naïve and myelin-immunized C57BL/6 mice were bled at the time points indicated and the frequency of peripheral blood monocytes was calculated based on the percentage of each monocyte subset multiplied by the total number of leukocytes per ml of blood (* p -
  Data is representative of three separate experiments.
  
  
  
  
  
  
• Figure S2. CD11b⁺CD115⁺Ly-6G⁻Ly-6C⁺ blood monocytes were FAC sorted from the peripheral blood of CFA immunized CD45.2⁺ mice (JPG, 49.4 KB) -
  5 × 10⁵ Ly-6C⁺CD45.2⁺ blood monocytes were intravenously injected into CD45.1⁺ mice at peak EAE. 48 hours later, CNS mononuclear cells of recipient animals were harvested and analyzed by flow cytometry using the markers shown. Data is representative of three independent experiments with similar results.