Blood, Vol. 113, Issue 16, 3813-3820, April 16, 2009

ICSBP-mediated immune protection against BCR-ABL–induced leukemia requires the CCL6 and CCL9 chemokines
Blood Nardi et al. 113: 3813

Supplemental materials for: Nardi et al

Files in this Data Supplement:

• Table S1. PCR primers used in this study (PDF, 20.1 KB)
  
  
• Figure S1. IFN alpha-mediated immunoprotection against BCR-ABL induced leukemia requires an intact immune system (JPG, 56.1 KB) -
  (A) IFN alpha mediated anti-leukemic protection does not occur in 6–9 week old Rag 2^−∕− injected with 1 × 10⁶ BaF3 cells expressing BCR-ABL and ICSBP (black square) or BCR-ABL and IFN alpha (grey diamond). (B) The IFN alpha mediated anti-leukemic protection occurs in 6–9 week old Balb/c mice injected with 1 × 10⁶ BaF3 cells expressing BCR-ABL and IFN alpha (black square) but is absent if the BCR-ABL–expressing cells carry IFN alpha (grey diamond) in a reverse orientation.
  
  
  
  
  
  
• Figure S2. ICSBP and Type I IFNs protect mice from leukemia cause by Imatinib-resistant mutated BCR-ABL (JPG, 75.8 KB) -
  (A) Survival curves for 6–9 week old Balb/c mice injected with 1 × 10⁶ BaF3 cells expressing L248R BCR-ABL variant with or without ICSBP, IFN alpha or IFN beta. (B) Survival curves for 6–9 week old Balb/c mice injected with 1 × 10⁶ BaF3 cells expressing the indicated BCR-ABL T315I variant with or without ICSBP, IFN alpha or IFN beta.
  
  
  
  
  
  
• Figure S3. Cluster representation of gene profiling data from parental BaF3 cells and BaF3 cells expressing BCR-ABL, ICSBP, or ICSBP and BCR-ABL (JPG, 114 KB) -
  1431 genes were organized into 15 different clusters based on their pattern of expression across the 4 cell types. Next to each cluster is described the effects of ICSBP and BCR-ABL on gene expression in each cluster, as well as the number of genes contained in each.
  
  
  
  
  
  
• Figure S4. Leukocytosis and relative lymphocytopenia in mice with BCR-ABL–induced leukemia and in mice that lose ICSBP-mediated anti-leukemic immune protection (JPG, 52 KB) -
  (A) Histogram representation of white blood counts (WBC) obtained from peripheral blood harvested from a Balb/c control mouse, and from mice injected with BaF3 cells expressing the indicated genes and shRNAs. Each experiment was repeated at least 3 times with n= 2 mice per condition. The normal WBC range of 1800–10700 cells/µl is indicated. (B) Histogram representation of percentage of lymphocytes obtained from the total white blood count performed on peripheral blood harvested from a Balb/c control mouse, and from mice injected with BaF3 cells expressing the indicated genes and shRNAs. The normal lymphocyte range of 55.8–95.6% is indicated.