Fig. S1. Cre-lox fate mapping during palate development reveals that Shh-expressing cells in the palate also contribute to taste buds. (A) Whole-mount intact palate stained with X-gal at E14.5 (when the palatal shelves (ps) are not yet fused) after E12.5 tamoxifen shows Shh descendent cells in the palatine rugae (arrows) of the anterior hard palate. (B) At E15.5 after E13.5 tamoxifen, Shh descendent cells are observed in the nasoincisor ducts (*), rugae (arrows) and the Geschmackstreifen (gss), each of which is a taste bud-bearing region in postnatal mice (Smith and Davis, 2000). (C) At P0, after tamoxifen on E12.5, Shh descendent cells are evident in the rugae and Gss. Scale bars: 100 m in A-C.