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Fig. S1. A representative fragment of the ZBP1 promoter with hypermethylated CpG sites in MTLn3 cells. Red C indicates methylated cytosines. Numbered positions are relative to the transcription start site.
Fig. S2. Immunofluorescence showing the cellular localization of β-catenin in breast non-tumorigenic MCF10 cells (average 100 cells were examined). β-catenin was not localized in cell nuclei (100%). Scale bar: 10 m.
Fig. S3. Extracts of MTLn3 and MTLn3-ZBP1 cells were fractionated in 10-50% linear sucrose gradients in the presence (A) or absence (B) of EDTA. Total RNA was extracted from the fractions and visualized by ethidium-bromide staining after electrophoresis (upper panels, respectively). Western blots show the distribution of ZBP1 from fractions of the sucrose gradients without or with EDTA (lower panels, respectively).