Supplementary material for Dollé et al. (2000) Proc. Natl. Acad. Sci. USA 97 (15), 8403-8408.

Table 2.

 Sequenced lacZ no-change mutations recovered from heart and small intestine of 3.5-month young and 32-month old mice

Heart

Small intestine

Base
position*

pUR288 sequence

Alteration

Base

position*

pUR288 sequence

Alteration

(5'®3')†

(5'®3')†

-------------------------------------- 3.5-month ---------------------------------------

285

GTTACC

C

AACTTA

C®T

325

CCAGCT

G

GCGTAA

G®A

810

TATCTG

G

AAGATC

G®T

692

TGCACT

G

GATGGT

G®A

1500

CTGACC

G

ATGATC

delG

692

TGCACT

G

GATGGT

G®A

2540

TAAAAA

A

CAACTG

delA

1387

GCTGTT

C

GCATTA

C®T‡

2540

TAAAAA

A

CAACTG

delA

1542

GTAACG

C

GAATGG

C®T‡

1006

GGCAGG

G

TGAAAC

G®TG

879

ACTACA

C

AAATCA

C®T

1178

CGACGG

C

ACGCTG

delC

1173

ACCGCC

G

ACGGCA

delG

1387

GCTGTT

C

GCATTA

C®T‡

1872

CTTTGC

G

AATACG

G®A‡

2179

TGCACT

G

GATGGT

G®A

2052

ACCGCA

C

GCCGCA

delC

2539

ATAAAA

AA

CAACTG

AA®T

2540

TAAAAA

A

CAACTG

delA

1108

TCGAAA

A

CCCGAA

delA

391

GCGAAT

G

GCGCTT

G®A

1226

GGTGCG

G

ATTGAA

delG

1043

TTTCGG

C

GGTGAA

delC

1290

CGTCAC

G

AGCATC

G®A‡

1772

GAAATG

G

TCCATC

G®A

1452

GTGGAT

G

AAGCCA

G®A

1994

TGATTT

T

GGCGAT

delT

2250

GGTAAA

C

AGTTGA

C®T

3005

CCCGTA

C

GTCTTC

delC

2373

TGGCAG

C

AGTGGC

C®T

748

CATTTT

T

ACGCGC

delT

939

GTACTG

G

AGGCTG

G®T

1396

ATTATC

C

GAACCA

C®T‡

1726

CGATGT

A

CGCGCG

A®C

1827

CTTTGC

G

AATACG

G®A‡

1827

CTTTGC

G

AATACG

G®A‡

1878

TACTGG

C

AGGCGT

C®T

1985

TTACGG

C

GGTGAT

delC

2250

GGTAAA

C

AGTTGA

C®T

2072

CTGTAT

G

AACGGT

delG

--------------------------------------- 32-month ---------------------------------------

621

TGGCTA

C

AGGAAG

C®T

1459

AAGCCA

A

TATTGA

A®T

1827

CTTTGC

G

AATACG

G®A‡

2098

AGTTTT

T

CCAGTT

delT

1827

CTTTGC

G

AATACG

G®A‡

2098

AGTTTT

T

CCAGTT

delT

1921

TCGTCT

G

GGACTG

G®T

2370

GCCTGG

C

AGCAGT

C®T

2098

AGTTTT

T

CCAGTT

delT

3005

CCCGTA

C

GTCTTC

C®A

1272

CTGATT

C

GAGGCG

C®T‡

1551

ATGGTG

C

AGCGCG

C®T

1387

GCTGTT

C

GCATTAT

C®T‡

1686

GGCGGC

G

GAGCCG

G®T

1832

CGAATA cgcccacgcgatgggta ACAGTC

17-bp del

1803

CCTGGA

G

AGACGC

G®T

2344

CATGGT

C

AGAAGC

C®G

1827

CTTTGC

G

AATACG

G®A‡

2540

TAAAAA

A

CAACTG

A®AA

1909

TACAGG

G

CGGCTT

G®A

3169

CATGGC

T

GAATAT

T®G

837

ATGAGC

G

GCATTT

G®A‡

819

GATCAG

G

ATATGT

G®T

1387

GCTGTT

C

GCATTAT

C®T‡

2283

CAGCCG

G

AGAGCG

G®T

2250

GGTAAA

C

AGTTGA

C®T

2499

AACCGC

C

AGTCAG

C®T

2535

GGCGAT

A

AAAAAC

A®T

2515

TTCTTT

C

ACAGAT

C®G

3108

TACAGT

C

AACAGC

C®T

3045

ACGCGC

G

AATTGA

G®T

1290

CGTCAC

G

AGCATC

G®A‡

663

AACTCG

G

CGTTTC

G®C

1387

GCTGTT

C

GCATTAT

C®T‡

816

GAAGAT

C

AGGATA

C®T

1846

CGATGG

G

TAACAG

delG

1589

CTGGTC

G

CTGGGG

G®TC

1982

GGCTTA

C

GGCGGT

C®T‡

1851

GGTAAC

A

GTCTTG

A

®C

2186

ACTGGATGGT ggcgctggatggt AAGCCG

13-bp del

2202

GGTAAG

C

CGCTGG

C

®G

2489

TTGGCA

A

TTTAAC

A

®C

2985

CCATTG

T

CAGACA

delT

The gray areas separate the mutants obtained from individual mice. The area with the black background indicates a triple mutant; all other mutants contained single mutations.

*

Nucleotide numbering according to SYNPUR288V (GenBank accession no. L09147).

†

Lowercase letters indicate deleted base sequence; underlined sequences indicate direct homology between breakpoints.

‡

G:C®A:T base change at CpG site.