Supplementary material for Angelastro et al. (2000) Proc. Natl. Acad. Sci. USA 97 (19), 10424–10429.

Fig. 3. Regulation of cofilin expression by nerve growth factor (NGF). (A) Northern blot analysis was carried out on total cellular RNA from naïve and NGF-treated (11 days) PC12 cells as described in Fig. 2. (B) Western immunoblotting of cofilin and actin-depolymerizing factor (ADF) in naïve and NGF-treated PC12 cells. Thirty micrograms of total cell protein was resolved on a 4-20% SDS/PAGE gel and transferred to nitrocellulose.