Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Pupo et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.180094797

Table 2.
PCR primers

Gene amplified	Primer (lab. no.)	Primer sequence (5'-3')	5' start position*	Amplicon
mdh	1406	TTCATCCTAAACTCCTTATT	37	975 bp
mdh	1407	TAATCGCAGGGGAAAGCAGG	1012	975 bp
argR	1404	TTCTTCAGCGTATCCAGCAT	-439	965 bp
argR	1405	GAGATGTGGTCATTGTAAAC	526	965 bp
thrB	1408	ATGAGCGTCGGGTTTGATGT	36	1,088 bp
thrB	1409	GGATTTCCTGTGGGATTTCA	1124	1,088 bp
thrC	1241	AGGGGCTGTTTTTTCCGCA	79	1,068 bp
thrC	1242	ATCGCTTCCACGCTCTCTT	1147	1,068 bp
trpC	1412	ACTGGATGACGACCAATA	419	998 bp
trpC	1413	ATCAGGATTTGTGGCACG	1417	998 bp
trpB	1314	TATTTTGGTGAGTTTGGCGG	21	1,167 bp
trpB	1911	TTCGTAGCGTTCCATCAGAT	1188	1,167 bp
purM	1246	CGTTTGCTTTCCCTGTTAGA	-72	1,061 bp
purM	1247	CAGAGGCTTTGATGATACCG	989	1,061 bp
purN	1410	AATGCCAACGGTGAAAACGC	-77	1,074 bp
purN	1411	GGAGCCTTGTTCTTCGCTAA	997	1,074 bp

*Designation of primer locations relative to the ATG start codon where the A is position 1 in the mdh, argR, thrB, thrC, trpB, trpC and purM sequences. In the case of the purN gene, the primer location is relative to the GTG start codon, where G is position 1. Note that the second primer listed for mdh, thrC, trpB, and purM is outside the gene to give overlap with the second amplicon of each segment.