Supplementary material for Pupo et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.180094797

Table 3.

Details of sequenced segments

Segment

Genes

Length, bp

Position*

GC

CAI

Gene name

EC Number

Function

thr

B-thrC
 

2,032§

0.1

     

thrB

867

64-930

0.57

0.37

Homoserine kinase

EC 2.7.1.39

Threonine biosynthesis

thrC

1,165

1-1165

0.53

0.33

Threonine synthase

EC 4.2.99.2

trp

B-trpC
 

1,486

28.3

     

trp

C

904

455-1358

0.53

0.32

N

-(5-phosphoribosyl) anthranilate isomerase, indole-3-glycerolphosphate synthetase

EC 4.1.1.48

Tryptophan synthesis

Intergenic

12

      

trp

B

570

1-570

0.56

0.42

Tryptophan synthase

EC 4.2.1.20

Tryptophan synthesis

pur

M-purN
 

2,101

56.2

     

Upstream

71

      

pur

M

1,038

1-1038

0.53

0.44

Phosphoribosylamino-imidazole synthetase

EC 6.3.3.1

Biosynthesis of purines

pur

N

639

1-639

0.54

0.35

5-Phosphoribosyglycinamide transformylase

EC 2.1.2.2

Downstream

354

      

mdh-arg

R
 

1,541

72.8

     

mdh

876

62-937

0.53

0.57

Malate dehydrogenase

EC 1.1.1.37

Citric acid cycle

Intergenic

431

      

arg

R

234

1-234

0.49

0.38

  

Regulation of arginine biosynthesis pathway

*For a segment, refers to the map position in minutes; for genes, refers the bases within a gene

The values for GC content and CAI are calculated from K12 sequence.

purM and purN overlap by 1 base.

§

For B13, only 1,082 bp (bases 84-1165) of the thrC gene were obtained, and the thrB gene was unable to be amplified.

Only 938 bp, including bp 62-937 of the mdh gene, were obtained for D8 and B13
.