Supplementary material for Pupo et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.180094797
Table 3.
Details of sequenced segmentsSegment | Genes | Length, bp | Position* | GC | CAI | Gene name | EC Number | Function |
thr B-thrC | 2,032§ | 0.1 | ||||||
thrB | 867 | 64-930 | 0.57 | 0.37 | Homoserine kinase | EC 2.7.1.39 | Threonine biosynthesis | |
thrC | 1,165 | 1-1165 | 0.53 | 0.33 | Threonine synthase | EC 4.2.99.2 | ||
trp B-trpC | 1,486 | 28.3 | ||||||
trp C | 904 | 455-1358 | 0.53 | 0.32 | N -(5-phosphoribosyl) anthranilate isomerase, indole-3-glycerolphosphate synthetase | EC 4.1.1.48 | Tryptophan synthesis | |
Intergenic | 12 | |||||||
trp B | 570 | 1-570 | 0.56 | 0.42 | Tryptophan synthase | EC 4.2.1.20 | Tryptophan synthesis | |
pur M-purN | 2,101 | 56.2 | ||||||
Upstream | 71 | |||||||
pur M | 1,038 | 1-1038 | 0.53 | 0.44 | Phosphoribosylamino-imidazole synthetase | EC 6.3.3.1 | Biosynthesis of purines | |
pur N | 639 | 1-639 | 0.54 | 0.35 | 5-Phosphoribosyglycinamide transformylase | EC 2.1.2.2 | ||
Downstream | 354 | |||||||
mdh-arg R | 1,541¶ | 72.8 | ||||||
mdh | 876 | 62-937 | 0.53 | 0.57 | Malate dehydrogenase | EC 1.1.1.37 | Citric acid cycle | |
Intergenic | 431 | |||||||
arg R | 234 | 1-234 | 0.49 | 0.38 | Regulation of arginine biosynthesis pathway |
*For a segment, refers to the map position in minutes; for genes, refers the bases within a gene
The values for GC content and CAI are calculated from K12 sequence.
purM and purN overlap by 1 base.§
For B13, only 1,082 bp (bases 84-1165) of the thrC gene were obtained, and the thrB gene was unable to be amplified.¶
Only 938 bp, including bp 62-937 of the mdh gene, were obtained for D8 and B13.