Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics -- Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Pupo et al., Proc. Natl. Acad. Sci. USA, 10.1073/pnas.180094797

Table 3.
Details of sequenced segments

Segment	Genes	Length, bp	Position*	GC^†	CAI^†	Gene name	EC Number	Function
thr B-thrC		2,032^§	0.1
	thrB	867	64-930	0.57	0.37	Homoserine kinase	EC 2.7.1.39	Threonine biosynthesis
	thrC	1,165	1-1165	0.53	0.33	Threonine synthase	EC 4.2.99.2	Threonine biosynthesis
trp B-trpC		1,486	28.3
	trp C	904	455-1358	0.53	0.32	N -(5-phosphoribosyl) anthranilate isomerase, indole-3-glycerolphosphate synthetase	EC 4.1.1.48	Tryptophan synthesis
	Intergenic	12
	trp B	570	1-570	0.56	0.42	Tryptophan synthase	EC 4.2.1.20	Tryptophan synthesis
pur M-purN		2,101	56.2
	Upstream	71
	pur M^‡	1,038	1-1038	0.53	0.44	Phosphoribosylamino-imidazole synthetase	EC 6.3.3.1	Biosynthesis of purines
	pur N^‡	639	1-639	0.54	0.35	5-Phosphoribosyglycinamide transformylase	EC 2.1.2.2	Biosynthesis of purines
	Downstream	354
mdh-arg R		1,541^¶	72.8
	mdh	876	62-937	0.53	0.57	Malate dehydrogenase	EC 1.1.1.37	Citric acid cycle
	Intergenic	431
	arg R	234	1-234	0.49	0.38			Regulation of arginine biosynthesis pathway

*For a segment, refers to the map position in minutes; for genes, refers the bases within a gene

^†The values for GC content and CAI are calculated from K12 sequence. ^‡ purM and purN overlap by 1 base. ^§For B13, only 1,082 bp (bases 84-1165) of the thrC gene were obtained, and the thrB gene was unable to be amplified. ^¶Only 938 bp, including bp 62-937 of the mdh gene, were obtained for D8 and B13.