J. Biol. Chem., Vol. 284, Issue 27, 17956-17967, July 3, 2009
Cdc50p Plays a Vital Role in the ATPase Reaction Cycle of the Putative Aminophospholipid Transporter Drs2p
J. Biol. Chem. Lenoir et al. 284: 17956
Guillaume Lenoir
Current Position: Associate Professor at the University of Paris-Sud 11, DSV/iBiTec-S/SB2SM in the Laboratory of Membrane Proteins at CEA Saclay in France
Education: Ph.D. in Biochemistry and Biophysics (2004) from Paris-Sud 11 University in France
Non-scientific Interests: Jazz-fusion music, hiking, guitar playing
I started working on membrane proteins during the internship of my master studies, and from that moment, a very intense relationship began. Despite quite some sweat and tears, I decided to pursue and join the Laboratory of Membrane Proteins at the CEA at Gif sur Yvette for my Ph.D. thesis. At the CEA, I set up a system for the heterologous expression and purification of a mammalian Ca2+-pump to allow a detailed analysis of its transport cycle. However, I found it odd to look at the function of a detergent-solubilized membrane protein without taking into account its natural lipid environment. In 2004, I decided to fix this problem by knocking at the door of the Department of Membrane Enzymology of the Institute of Biomembranes in Utrecht, The Netherlands. There, the research topics are related to protein/lipid interactions in the dynamic organization of cellular membranes. I began working as a postdoctoral associate in the group of Joost Holthuis on a Ca2+-pump-related class of proteins that catalyze lipid transport across cellular bilayers (so-called "flippases"). Thanks to a very inspiring environment, I uncovered a vital role for an accessory protein, named Cdc50p, in the transport cycle of the putative lipid flippase Drs2p. We propose that Cdc50 proteins are integral components of the flippase machinery. I am now back in the Laboratory of Membrane Proteins in France, where I aim to further elucidate the transport mechanism of flippases, using biochemical and biophysical approaches.
Read Dr. Lenoir's article on page 17956.
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