Blood, Vol. 113, Issue 26, 6648-6657, June 25, 2009

microRNA 184 regulates expression of NFAT1 in umbilical cord blood CD4⁺ T cells
Blood Weitzel et al. 113: 6648

Supplemental materials for: Weitzel et al

Files in this Data Supplement:

• Figure S1. Diagram of the 3′ UTR luciferase constructs utilized in Fig. 4 and sequence inserted into the vector cloning site (JPG, 85.6 KB) -
  
  
  
  
  
  
• Figure S2. CD14^–4⁺ T cells were isolated from UCB and AB samples and stimulated as described (JPG, 58.9 KB) -
  Whole cell lysates from 5 samples of each type were pooled and separated via centrifugation through a 10−50% sucrose gradient and fractions collected. Each fraction was spiked with a consistent quantity of non-interfering mouse RNA to control for RNA recovery. Human NFATc2 and Mouse GAPDH were assayed via quantitative RTPCR for each fraction as described. NFATc2 expression was expressed relative to the mouse GAPDH and each data point presented as relative to the highest NFATc2-expressing fraction.
  
  
  
  
  
  
• Figure S3. Unfractionated CD14^–4⁺ T cells and CD45RA⁺ naïve T cells were isolated from AB and UCB samples and miR-184 was quantified via RT-PCR as described (JPG, 49.2 KB) -
  Expression of miR-184 in the CD45RA⁺ cells was normalized to expression of the unfractionated CD4⁺ cells from the same sample. Data indicates much stronger skewing towards miR-184 expression primarily in naïve CD45RA⁺ T cells in AB than in UCB.