Fig. S1. Role of IQGAP1 in ECM migration: wound-healing assay. (A) Quantification of the distances migrated by NIH3T3 cell lines expressing IQGAP1-mutants after 12 hours of wounding. The distance between the wound edges were measured and calculated as a percentage of the distance at day 0 (100%) from three independent experiments with the error bars representing means ± s.e.m. (P<0.005). (B) Wound-healing assay of IQGAP1-mutant cells 24 hours after wounding. Confluent monolayer (at ∼98%) were scratched with a sterile 200 l pipette tip and photographed (immediately: day zero, see Fig. 7) and again after 24 hours. (C) Expression of IQGAP1-F leads to β-catenin translocation to the nucleus, which is inhibited by IQGAP1-C or IQGAP1-N. Stable NIH3T3 cells were untreated or treated with EGF for 5 minutes, fixed and stained with β-catenin antibodies. The cells shown in the micrographs are representative of >90% of the cells in each condition.