USER Friendly Cloning Coupled with Chitin-Based Natural Transformation Enables Rapid Mutagenesis of Vibrio vulnificus
Appl. Environ. Microbiol. Gulig et al. 75: 4936

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Construction of pCVD442 and pCOS5 plasmid derivatives for USER friendly cloning (Fig. S1).
    PDF file, 132K.
  • Supplemental file 2 - Construction of an allelic-exchange plasmid for deletion of flaFBA by TOPO TA cloning and subcloning (Fig. S2).
    PDF file, 132K.
  • Supplemental file 3 - Construction of an allelic-exchange plasmid for deletion of rtxA1 by three-part USER friendly cloning (Fig. S3).
    PDF file, 123K.