Blood -- Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras

Blood, Vol. 114, Issue 7, 1445-1453, August 13, 2009

Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras
Blood Sarzotti-Kelsoe et al. 114: 1445

Supplemental materials for: Sarzotti-Kelsoe et al

Files in this Data Supplement:

Figure S1. Spectratype analysis of the TCR Vβ repertoire using two methods (JPG, 67.6 KB) -
Example of the conversion from the previously used classification of TCR Vβ peak profiles as oligoclonal, polyclonal skewed, and polyclonal Gaussian into DKL values, using the same experimental results. DKL values of 0.41 correspond to 75% oligoclonal, 25% polyclonal skewed, DKL of 0.06 to 50% polyclonal skewed and 50% polyclonal Gaussian, DKL of 0.02 to 4% polyclonal skewed and 96% polyclonal Gaussian.
Figure S2. Longitudinal analysis of thymic output in three SCID patients previously reported (JPG, 33.1 KB) -
J-1, J-2 and X-5, were studied for TREC analysis beyond the time point previously reported (indicated by the arrows). All three patients demonstrated a sustained thymic function (1,000-10,000 TREC/µg DNA) for ≥14 years post transplant.