Blood, Vol. 114, Issue 7, 1374-1382, August 13, 2009

Src homology 2 domain–containing inositol-5-phosphatase and CCAAT enhancer-binding protein β are targeted by miR-155 in B cells of Eµ-MiR-155 transgenic mice
Blood Costinean et al. 114: 1374

Supplemental materials for: Costinean et al

Files in this Data Supplement:

• Figure S1. Both strains, C57BL/6 and FVB, presented the same phenotype (JPG, 167 KB) -
  (A) C57BL/6 transgenic mouse no. 317 with prominent splenomegaly (* indicates the enlarged spleen), dead of leukemia; (B) FVB mouse no. 764 displaying an identical phenotype; (C) Sick miR155 transgenic mouse (FVB strain) exhibiting hunched posture and ruffled fur; (D) Enlarged spleen of the FVB mouse no. 764.
  
  
  
  
  
  
• Figure S2. CD4 and CD8 expression assessed by flow cytometry in the transgenics compared to the wild type mice (JPG, 166 KB) -
  
  
  
  
  
  
• Figure S3. V(D)J rearrangement probed with a JH4 probe (Southern Blot) (JPG, 224 KB) -
  (A) Oligo/monoclonal rearrangement of the B-cell miR 155 leukemias (small black arrows indicate bands of oligo/monoclonal rearrangement in the transgenics compared to the wild types; red arrow indicates germ line band; blue arrow indicates a cross hybridizing normal band) — the genomic DNA was digested with PvuII (see materials and methods); (B) Same analysis as in (A) shows in lane 1 a leukemia with a few rearranged clones while in lane 3 there is only one rearranged band (see blue asterisk); leukemia in lane 2 is still polyclonal; the red asterisk indicates the germ line; the genomic DNA was digested with EcoRI; M = DNA molecular marker.
  
  
  
  
  
  
• Figure S4. Base complementarity between Ship 3′-UTR and miR 155 “seed” sequence (JPG, 41.9 KB) -
  
  
  
  
  
  
• Figure S5. Base complementarity between C/ebpβ 3′-UTR and miR 155 “seed” sequence (JPG, 43.4 KB) -