Files in this Data Supplement:
Movie 1. Confocal stacked image of ERK5-GFP plus empty vector. C2 myoblasts were co-transfected with empty vector and ERK5-GFP and after 24 hours in GM were transferred to GM for 10 minutes. GFP fluorescence (green) was captured in successive confocal slices through cells to show ERK5 localisation (green), or nuclear staining using DAPI (blue); magnification 1000×; representative image from n=4.
Movie 2. Confocal stacked image of ERK5AEF-GFP plus empty vector. C2 myoblasts were co-transfected with empty vector and ERK5AEF-GFP and after 24 hours in GM were transferred to GM for 10 minutes. GFP fluorescence (green) was captured in successive confocal slices through cells to show ERK5 localisation (green), or nuclear staining using DAPI (blue); magnification 1000×; representative image from n=4.
Movie 3. Confocal stacked image of ERK5-GFP plus MEK5DD. C2 myoblasts were co-transfected with MEK5DD and ERK5-GFP and after 24 hours in GM were transferred to GM for 10 minutes. GFP fluorescence (green) was captured in successive confocal slices through cells to show ERK5 localisation (green), or nuclear staining using DAPI (blue); magnification 1000×; representative image from n=4.
Movie 4. Confocal stacked image of ERK5AEF-GFP plus MEK5DD. C2 myoblasts were co-transfected with MEK5DD and ERK5AEF-GFP and after 24 hours in GM were transferred to GM for 10 minutes. GFP fluorescence (green) was captured in successive confocal slices through cells to show ERK5 localisation (green), or nuclear staining using DAPI (blue); magnification 1000×; representative image from n=4.