Targeted Overexpression of EZH2 in the Mammary Gland Disrupts Ductal Morphogenesis and Causes Epithelial Hyperplasia
Am J Pathol Li et al. 175: 1246
Supplemental Material
Thus supplemental Material includes supporting data for this study.
Files in this Data Supplement:
- Supplemental Figure 1 - EZH2 overexpression results in hyperplasia of differentiated luminal cells. Immunohistochemical staining for the differentiated luminal cell markers cytokeratin 18 and E-cadherin in mature ducts of virgin wild-type and MMTV-EZH2 of 4 months of age.
- Supplemental Figure 2 - EZH2 overexpression does not influence apoptosis during involution. Bar graph of TUNEL assay results at 1, 3, 4, 7 and 14 days after forced weaning of lactation. As expected, the percentage of apoptotic cells increased in the early phases of involution. To be able to accurately determine the number of apoptotic cells, we have normalized the tunnel positive cells to the number of epithelial cells in each microscopic field. We counted 10, 40X fields per slide from 5 EZH2 transgenic mice and 5 wild-type mice. Error bars=SD.
- Supplemental Figure 3 - EZH2 overexpression up-regulates ƒÒ-catenin messenger RNA in the mammary gland. Real-time PCR analysis showed £-catenin expression level in transgenic and wild-type mice mammary glands relative to GAPDH and normalized to the wild-type. 5 w: 5 week, 4 m: 4 month, L: lactating, and 4 i: involution day 4. Samples were prepared in quadruplicate and the experiment was repeated three times. (*Student¡¦s t test