Fig. S1. Endogenous PBF protein in FRTL-5 cells. Immunofluorescent staining of endogenous PBF using the rabbit polyclonal anti-PBF antibody. Inset: Following immunoblotting with rabbit polyclonal anti-PBF antibody, a band corresponding to PBF was observed at approximately 30 kDa in lysate harvested from FRTL-5 cells. Scale bar: 20 M.

Fig. S2. Immunofluorescent detection of PBF-HA. PBF-HA was transiently transfected into both COS-7 cells (A) and FRTL-5 cells (B) and stained with the mouse monoclonal anti-HA.11 antibody (green in successfully transfected cells) and rabbit polyclonal anti-PBF antibody (red). Yellow staining in the merged images on the right demonstrated concomitant staining of PBF with both antibodies. Scale bars: 20 M.

Fig. S3. Transfection efficiency was determined to be ∼15-25% in FRTL-5 cells by transiently transfecting a GFP plasmid. (A) Representative image of GFP and Hoechst-stained nuclei obtained using conventional microscopy (×100 magnification). (B) GFP fluorescence shown overlaying light microscopy image of the cells (×40 magnification). Scale bars: 20 M.