Complete WO Phage Sequences Reveal Their Dynamic Evolutionary Trajectories and Putative Functional Elements Required for Integration into the Wolbachia Genome -- Tanaka et al. 75 (17): 5676 Data Supplement - Supplemental material -- Applied and Environmental Microbiology

Complete WO Phage Sequences Reveal Their Dynamic Evolutionary Trajectories and Putative Functional Elements Required for Integration into the Wolbachia Genome
Appl. Environ. Microbiol. Tanaka et al. 75: 5676

Supplemental material

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Supplemental file 1 - Supplemental text and references; life cycle of typical bacteriophages alternating between lytic phase and lysogenic phase (Fig. S1); structural comparison between the WOcauB2 and WOcauB3 prophage sequences (Fig. S2) and between the WOcauB2 prophage sequence and the WOcauB1 sequence (Fig. S3); alignment of WOcauB1 and WOcauB2 sequences around the discrepant region (Fig. S4); molecular phylogenetic analysis of 15 gene product sequences encoded by the WO prophages from Wolbachia strains wCauB, wMel, wPip, and wKue (Fig. S5); phylogenetic relationship of the integrase gene products encoded in WO prophages on the basis of the conservative catalytic domain sequence (Fig. S6); estimated core sequences for WOcauB2 and WOcauB3 (Fig. S7); genes with similarity to B3gp45 of WOcauB3 in the databases (Fig. S8) and to GF2gp17 of wCauB-GF2 in the databases (Fig. S9); structural comparison of diverse patatin-like proteins (Fig. S10).
List of ORFs encoded in WOcauB2 and WOcauB3 prophages determined in this study (Table S1), encoded in wCauB genome fragment 1 that flank WOcauB2 prophage (Table S2), and encoded in wCauB genome fragment 2 that flank WOcauB3 prophage (Table S3); PCR-RFLP detection of WOcauB1-type and WOcauB2-type gene sequences from the Wolbachia strain wCauB infection E. kuehniella (Table S4).
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