Low grade inflammation and coronary heart disease: prospective study and updated meta-analyses -- Danesh et al. 321 (7255): 199 Data Supplement

Table A

Table A Comparisons of levels of risk factors and other characteristics in controls by thirds of C reactive protein concentrations. Values are mean (SD) unless stated otherwise

	Top (>2.4 mg/l)	Middle (0.9-2.4 mg/l)	Bottom (<0.9 mg/l)	t value†	t value‡
No of men	347	298	380
Age (years)	53.1 (5.1)	52.1 (5.3)	51.3 (5.5	4.7	2.7*
No (%) current smokers	195 (56)	126 (42)	115 (30)	7.4	7.4***
No (%) >2 drinks alcohol/day	90 (26)	74 (25)	67 (18)	1.7	1.8
Physical measurements
Body mass index (kg/m²)	26.2 (3.4)	25.4 (3.2)	24.3 (3.0)	7.7	9.1***
Height (cm)	172.6 (6.3)	172.8 (6.7)	173.2 (6.6)	1.3	0.6
Weight (kg)	78 (12)	76 (11)	73 (10)	6.1	7.4***
Systolic blood pressure (mmHg)	149 (20)	148 (22)	143 (20)	1.9	1.8
Diastolic blood pressure (mmHg)	83 (12)	84 (14)	81 (13)	1.9	1.3
Forced expiratory volume (l)	298 (74)	326 (75)	350 (71)	8.3	6.9***
Plasma acute phase reactants
Log₁₀ serum amyloid A protein (mg/l)	1.01 (0.32)	0.83 (0.21)	0.69 (0.26)	24	17***
Albumin (g/l)	44.0 (2.6)	44.8 (2.5)	44.8 (2.2)	5.9	3.4**
Leucocyte count (×10⁹)	7.8 (1.9)	7.2 (1.7)	6.6 (1.5)	10.7	7.6***
Other blood analytes
No (%) H pylori seropositive	270 (78)	219 (73)	251 (66)	2.3	1.0
C pneumoniae titre (FC×10⁶)	189 (58)	183 (58)	172 (62)	2.8	2.0
Total cholesterol (mmol/l)	6.29 (1.06)	6.25 (0.95)	6.07 (0.94)	2.7	1.9
HDL cholesterol (mmol/l)	1.10 (0.26)	1.18 (0.33)	1.18 (0.27)	5.0	2.6*
Triglyceride (mmol/l)	2.08 (1.21)	1.93 (1.22)	1.80 (1.10)	3.1	0.9
Homocysteine (µmol/l)	15.3 (9.0)	15.4 (10.1)	14.3 (8.5)	0.7	0.7
Packed cell volume (%)	42.8 (9.9)	43.4 (6.9)	41.9 (8.5)	0.3	0.3
Glucose (mmol/l)	5.7 (1.5)	5.6 (1.5)	5.6 (1.1)	0.7	0.5
Urea (mmol/l)	5.3 (1.4)	5.5 (1.3)	5.4 (1.4)	1.7	1.3
Urate (mmol/l)	360 (69)	358 (64)	337 (65)	4.3	2.7*
Creatinine (µmol/l)	99 (14)	100 (13)	98 (13)	0.3	0.8
Insulin (mU/l)	20 (20)	15 (16)	15 (14)	3.2	1.4

†Derived from regression of C reactive protein values on each characteristic separately adjusting for age and town only.

‡Derived from regression of C reactive protein values adjusting for age, town, smoking, body mass index, and markers of socioeconomic status (including height).

*P<0.01,**P<0.001,***P<0.0001.

FC=fluoresecent count.

Table B Comparisons of levels of risk factors and other characteristics in controls by thirds of serum amyloid A protein concentrations. Values are mean (SD) unless stated otherwise

	Top (>9 mg/l)	Middle (6-9 mg/l)	Bottom (<6 mg/l)	t value†	t value‡
No of men	362	351	312
Age (years)	52.7 (5.3)	52.1 (5.3)	51.5 (5.5)	2.9	1.6
No (%) current smokers	164 (46)	136 (39)	136 (44)	1.5	3.2*
No (%) >2 drinks alcohol/day	83 (23)	79 (23)	69 (22)	1.1	0.5
Physical measurements
Body mass index (kg/m²)	26.0 (3.3)	25.5 (3.1)	24.3 (3.3)	4.9	5.5***
Height (cm)	173 (6)	173 (7)	173 (7)	0.1	0.5
Weight (kg)	78 (11)	76 (10)	73 (11)	4.3	4.4***
Systolic blood pressure (mmHg)	147 (21)	148 (21)	145 (20)	0.2	0.1
Diastolic blood pressure (mmHg)	84 (14)	83 (13)	81 (13)	0.6	0.0
Forced expiratory volume (l)	314 (76)	330 (74)	335 (78)	4.0	3.4**
Plasma acute phase reactants
Log₁₀ C reactive protein (mg/l)	0.47 (0.51)	0.09 (0.43)	0.14 (0.46)	24	17***
Albumin (g/l)	44.2 (2.7)	44.8 (2.3)	44.6 (2.2)	4.5	1.8
Leucocyte count (×10⁹/l)	7.6 (1.9)	7.0 (1.6)	6.9 (1.7)	6.1	4.9***
Other blood analytes
No (%) H pylori seropositive	261 (72)	258 (74)	221 (71)	0.0	0.2
C pneumoniae titre (FC×10⁶)	185 (60)	181 (60)	176 (60)	1.1	0.1
Total cholesterol (mmol/l)	6.42 (1.08)	6.26 (0.91)	5.88 (0.89)	5.3	4.8***
HDL cholesterol (mmol/l)	1.12 (0.28)	1.14 (0.28)	1.20 (0.30)	4.1	1.5
Triglyceride (mmol/l)	1.94 (1.18)	2.00 (1.13)	1.74 (1.25)	2.2	1.2
Homocysteine (µmol/l)	15.6 (10.7)	14.3 (7.9)	15.0 (8.9)	1.0	1.2
Packed cell volume (%)	42.9 (9.2)	43.0 (7.6)	41.9 (9.0)	0.5	0.3
Glucose (mmol/l)	5.7 (1.5)	5.6 (1.0)	5.7 (1.5)	0.5	0.3
Urea (mmol/l)	5.5 (1.4)	5.4 (1.3)	5.3 (1.4)	1.4	1.5
Urate (mmol/l)	358 (70)	349 (62)	344 (68)	1.8	0.2
Creatinine (µmol/l)	100 (15)	99 (13)	98 (13)	1.2	1.7
Insulin (mU/l)	18 (20)	17 (14)	15 (17)	1.7	0.4

†Derived from regression of serum amyloid A protein C reactive protein on each characteristic separately adjusting for age and town only.

‡Derived from regression of serum amyloid A protein values adjusting for age, town, smoking, body mass index, and markers of socioeconomic status (including height).

*P<0.01,**P<0.001,***P<0.0001.

Table C Comparisons of levels of risk factors and other characteristics in controls by thirds of leucocyte count. Values are mean (SD) unless stated otherwise

	Top (>7.8×10⁹/l)	Middle (6.4-7.8×10⁹/l)	Bottom (<6.4×10⁹/l)	t value†	t value‡
No of men	332	329	330
Age (years)	52.4 (5.2)	52.0 (5.4)	52.0 (5.4)	0.2	1.4
No (%) current smokers	214 (65)	136 (41)	70 (21)	11.4	10.6***
No (%) >2 drinks alcohol/day	81 (24)	77 (23)	70 (21)	0.7	0.6
Physical measurements
Body mass index (kg/m²)	25.5 (3.4)	25.3 (3.4)	25.2 (3.1)	1.0	1.9
Height (cm)	172 (6)	173 (7)	173 (7)	1.8	1.7
Weight (kg)	76 (11)	76 (12)	76 (11)	0.0	0.7
Systolic blood pressure (mmHg)	147 (21)	147 (22)	146 (20)	0.5	0.3
Diastolic blood pressure (mmHg)	81 (13)	83 (13)	83 (13)	2.6	1.5
Forced expiratory volume (l)	311 (77)	327 (77)	342 (73)	5.1	2.6*
Plasma acute phase reactants
Log₁₀ C reactive protein (mg/l)	0.34 (0.50)	0.15 (0.50)	0.04 (0.50)	10.7	7.6***
Log₁₀ serum amyloid A protein (mg/l)	0.89 (0.32)	0.84 (0.26)	0.78 (0.29)	6.1	4.9***
Albumin (g/l)	44.5 (2.5)	44.4 (2.6)	44.9 (2.2)	1.3	1.1
Other blood analytes
No (%) H pylori seropositive	254 (77)	250 (76)	210 (64)	2.9	1.3
C pneumoniae titre (FC×10⁶)	187 (61)	184 (57)	172 (60)	2.9	3.2*
Total cholesterol (mmol/l)	6.29 (1.01)	6.20 (0.99)	6.13 (0.96)	1.4	1.6
HDL cholesterol (mmol/l)	1.13 (0.27)	1.14 (0.32)	1.19 (0.27)	2.6	2.1
Triglyceride (mmol/l)	2.05 (1.32)	2.00 (1.18)	1.74 (0.96)	3.1	1.8
Homocysteine (µmol/l)	15 (10)	15 (9)	14 (7)	0.2	0.7
Packed cell volume (%)	45.0 (3.3)	44.2 (3.1)	43.2 (3.0)	8.3	5.5***
Glucose (mmol/l)	5.6 (1.7)	5.6 (1.2)	5.6 (1.1)	0.9	0.6
Urea (mmol/l)	5.3 (1.4)	5.5 (1.4)	5.5 (1.3)	1.3	0.5
Urate (mmol/l)	349 (68)	355 (66)	350 (66)	0.0	0.4
Creatinine (µmol/l)	97 (13)	100 (13)	99 (14)	0.8	0.1
Insulin (mU/l)	16 (16)	18 (20)	15 (15)	0.7	0.6

†Derived from regression of the leucocyte count C on each characteristic separately adjusting for age and town only.

‡Derived from regression of the leucocyte count adjusting for age, town, smoking, body mass index, and markers of socioeconomic status (including height).

*P<0.01,**P<0.001,***P<0.0001.

Table D Comparisons of levels of risk factors and other characteristics in controls by thirds of albumin concentrations. Values are mean (SD) unless stated otherwise

	Top (>46 g/l)	Middle (44-46 g/l)	Bottom (<6.4 g/l)	t value†	t value‡
No of men	348	347	327
Age (years)	51.2 (5.4)	52.3 (5.3)	53.0 (5.3)	6.0	4.1***
No (%) current smokers	120 (34)	131 (38)	183 (56)	5.0	4.3***
No (%) >2 drinks alcohol/day	89 (26)	75 (22)	67 (21)	1.6	1.9
Physical measurements
Body mass index (kg/m²)	25.6 (3.2)	25.5 (3.0)	24.9 (3.7)	3.3	1.1
Height (cm)	173 (7)	173 (7)	173 (6)	0.6	0.7
Weight (kg)	77 (11)	76 (10)	74 (13)	3.1	0.6
Systolic blood pressure (mmHg)	149 (21)	148 (21)	143 (21)	6.7	5.3***
Diastolic blood pressure (mmHg)	84 (13)	83 (13)	80 (13)	5.8	3.9***
Forced expiratory volume (l)	337 (74)	329 (74)	311 (79)	3.9	2.5
Plasma acute phase reactants
Log₁₀ C reactive protein (mg/l)	0.06 (0.48)	0.11 (0.50)	0.30 (0.58)	5.9	3.4**
Log₁₀ serum amyloid A protein (mg/l)	0.81 (0.26)	0.82 (0.26)	0.90 (0.36)	4.5	1.8
Albumin (g/l)	7.1 (1.7)	7.1 (1.9)	7.4 (1.7)	1.3	1.1
Other blood analytes
No (%) H pylori seropositive	225 (65)	249 (72)	262 (80)	3.1	1.4
C pneumoniae titre (FC×10⁶)	176 (60)	184 (60)	184 (60)	0.9	1.1
Total cholesterol (mmol/l)	6.47 (0.96)	6.20 (0.97)	5.90 (0.97)	8.9	6.9***
HDL cholesterol (mmol/l)	1.18 (0.27)	1.16 (0.32)	1.12 (0.27)	4.3	3.2*
Triglyceride (mmol/l)	2.18 (1.39)	1.87 (1.10)	1.75 (0.95)	3.6	2.5
Homocysteine (µmol/l)	14 (8)	16 (11)	15 (8)	1.1	0.6
Packed cell volume (%)	44.3 (5.5)	42.7 (8.4)	41.0 (11)	4.8	4.5***
Glucose (mmol/l)	5.7 (1.6)	5.6 (1.1)	5.6 (1.4)	0.9	0.9
Urea (mmol/l)	5.2 (1.4)	5.5 (1.4)	5.2 (1.3)	3.7	2.7*
Urate (mmol/l)	358 (68)	351 (71)	344 (61)	4.5	2.8*
Creatinine (µmol/l)	100 (14)	99 (13)	97 (14)	2.6	0.5
Insulin (mU/l)	17 (18)	16 (16)	16 (17)	1.2	1.8

†Derived from regression of albumin values on each characteristic separately adjusting for age and town only.

‡Derived from regression of albumin values adjusting for age, town, smoking, body mass index, and markers of socioeconomic status (including height).

*P<0.01,**P<0.001,***P<0.0001.