Supplementary Materials
Supplemental Materials and Methods
Western Blotting
Western blots were performed on dorsal striatal tissue samples obtained in parallel with those used for the adenylyl cyclase assays. Briefly, BCA assay (Pierce, Rockford, IL, USA) was used to determine protein concentrations of each sample, and equal amounts of protein were loaded in a 10% SDS-polyacrylamide gel. Equal protein loading was also verified by visualization of total protein by Ponceau Red after transfer to nitrocellulose and/or probing the membranes with anti-actin antibody (1:500, Sigma, St. Louis, MO, USA). DARPP-32 antibody (generously provided by Dr. Hugh Hemmings, The Rockefeller University, New York, NY, USA) was used in a concentration of 1:10,000, Erk1/2 antibody (Transduction Labs, Lexington, KY, USA) was used in a concentration of 1:1000. Blots were reacted with NEN-DuPont chemiluminescence system (Boston, MA, USA), and exposed to film. Densitometric values were obtained using Bio-Soft Scananalysis for Apple (Biosoft, Ferguson, MO, USA).
Supplemental Results
ERK and DARPP-32 protein 
Western blot analysis was used to determine the levels of ERK and DARPP-32 protein in the striatum of adult mice exposed prenatally to cocaine or saline. No differences between prenatal treatment groups were revealed for any of the three proteins measured from dorsal striatal extracts (data not shown).

Supplementary Table 1. Prenatal cocaine and saline exposure gestation.
Prenatal Treatment	Average Weight Gain of Dams (g)	Average number of live pups per litter	P15	Weight (g)   P36	P60	
PCOC40	57.7	11	8.824	30.515	39.066	
PSAL	64.5	11	9.058	30.789	36.756	
Average	61.1	11	8.941	30.656	37.876	

Supplementary Table 1. Dam weight and the number of live pups born to each dam were recorded daily. No effect of prenatal treatment (cocaine [PCOC40] or saline [PSAL]) on the average number of pups per litter was found. All mice gained weight with age, however this was unaffected by prenatal treatment. Also, the percent gestational weight gain of the dam was unaffected by the prenatal treatment.

Supplemental Figure 1.  Basal, forskolin- and D1 agonist-stimulated adenylyl cyclase activity in the striatum of adult mice prenatally exposed to cocaine. Values (mean + SEM) are given for female (N=9-12) mice that were exposed in utero to saline (PSAL) or cocaine (40 mg/kg/day PCOC40. Data are expressed in percent of saline controls. A. Neither basal nor forskolin-stimulated adenylyl cyclase activity was significantly different between treatment groups. B. Females exposed in utero to 40 mg/kg/day cocaine showed no difference in D1 receptor-stimulated adenylyl cyclase activity compare to the saline-exposed control group.