Augmentation of Cav1 channel current and action potential duration after uptake of S100A1 in sympathetic ganglion neurons -- Hernández-Ochoa et al. 297 (4): C955 Data Supplement - Supplemental Figures and Video -- AJP

Supplemental Figures and Video

2 figures in PDF format; one video file in AVI format.

Files in this Data Supplement:

Figure S1 - Nifedipine prevents the effect of S100A1 on Ca2+ currents. A, Representative Ca2+ channel current traces obtained at different times (3, 5 and 8 min) after the breakthrough into the whole-cell configuration for S100A1-dialyzed neurons (A) and for neurons pre-treated with nifedipine and then dialyzed with S100A1 (B). Current was elicited using a step depolarization to 0 mV.
Figure S2 - Representative transmitted light (A, C) and confocal (B, and D-G) images of a 48h cultured SCG neuron before (A, B C and D) and 3 (E), 5 (F) and 10 min (G) after breakthrough into the whole-cell configuration and dialysis with S100A1-alexa488. (H) Bar plot indicating calculated intracellular S100A1-alexa488 concentration at different times after breakthrough into whole-cell configuration. Scale bar 20 μm.
Video File - Frequently, in mass cultures of SCG neurons, S100A1-alexa488 punctate fluorescent signals within cell bodies and axons were transported in both an anterograde and retrograde fashion. Time-lapse xy confocal images of the labeled cells revealed movement (0.4 μm/s) of small fluorescent particles within cell bodies and along neuronal processes.