Blood, Vol. 114, Issue 18, 3783-3792, October 29, 2009

Kinetics of normal hematopoietic stem and progenitor cells in a Notch1-induced leukemia model
Blood Hu et al. 114: 3783

Supplemental materials for: Hu et al

Files in this Data Supplement:

• Table S1. Limited regeneration of primitive cells in leukemic marrow (PDF, 17.8 KB)
  
  
• Table S2. The sequences of primers for the selected cell cycle genes (PDF, 65 KB)
  
  
• Figure S1. Immuno-phenotype of the Notch-induced T-ALL in the affected mice shown by flow cytometry (JPG, 260 KB) -
  
  
  
  
  
  
• Figure S2. HSC enumeration with the SLAM markers (JPG, 435 KB) -
  A similar transplant experiment as shown in Fig. 1a was performed except for the fact that a higher dose of normal competitor BMNCs from C57BL/6J (1 × 10⁸, CD45.2⁺) was co-transplanted with 1 × 10⁷ BMNCs from B6.SJL (CD45.1⁺) into lethally-irradiated recipients (A). At day 14 after transplantation, BMNCs from leukemic hosts or control were analyzed for the expression of CD45.1, CD150, CD48, c-Kit, Sca-1, and lineage markers with flow cytometry. Representative flow profiles and the average frequency of CD45.1⁺CD150⁺CD48⁻LKS cells in leukemic or control hosts are shown (B). Each value is mean ± s.d.. ** P
  
  
  
  
  
• Figure S3. Annexin-V staining (JPG, 373 KB) -
  BMNCs were stained with lineage antibodies and Annexin-V. Lin⁻ cells were gated by means of a stringent parameter. The representative plot is shown above. The average frequency of the (Annexin V⁺PI⁻) fraction in CD45.1⁺Lin⁻ cells from leukemic or control groups is shown (2 experiments, 5 mice/group in each experiment, P=0.48).
  
  
  
  
  
  
• Figure S4. Proposed models for the reversible inhibition of hematopoietic stem cells in the leukemic microenvironment (JPG, 535 KB) -