Data Supplement
Files in this Data Supplement:
- Data Supplement - Supplemental Figure 1. Capsaicin (300 nM) pretreatment preserves KCl stimulated FM1-43 labeling and reduces BoNT/A uptake into Neuro 2a cells. A. Confocal images of FM 1-43 labeling for control (left), BoNT/A alone (middle), and 300 nM capsaicin pretreated (right) Neuro 2a cells. B. Representative fluorescence labeling of Neuro 2a cells with alexa 647-BoNT/A for BoNT/A alone (A) and BoNT/A after capsaicin (500 nM) pretreament (B). BoNT/A was loaded by incubating cells with alexa 647-BoNT/A as described in the methods. The right panels present corresponding bright field images.