Temporal and spatial dissection of Shh signaling in genital tubercle development -- Lin et al. 136 (23): 3959 Data Supplement - DEV039768 Supplementary Material -- Development

DEV039768 Supplementary Material

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Supplemental Figure S1 -
Fig. S1. Shh regulates the expression of Hoxa13 and Hoxd13. (A,B) Hoxd13 whole-mount in situ hybridization on Shh-cKO and wild-type limb and GT showing comparable expression in the limb but reduced expression in the GT. (C,D) Real-time RT-PCR analysis showing downregulation of both Hoxa13 (C) and Hoxd13 (D) in the Shh-cKO GT.
Supplemental Figure S2 -
Fig. S2. Defective cloaca septation in Shh and Smo mutants. (A-D) H&E staining on E12.5 mid-sagittal sections of embryos of the indicated genotype. Both Shh-cKO (B) and Mes-Smo-cKO (C) show defective cloaca septation (arrows in B,D), whereas UE-Smo-cKO shows complete separation of urogenital sinus and anal channel (C).
Supplemental Figure S3 -
Fig. S3. Gene expression analysis in UE-Smo-cKOs and UEmes-Smo-cKOs. (A-F) Whole-mount in situ analysis on E12.5 UE-Smo-cKOs (D-F) and controls (A-C) revealed no difference in expression of Fgf8, Hoxd13 and Hoxa13. (G-N) Whole-mount in situ analysis on E9.5 Dox-treated E12.5 UEmes-Smo-cKOs (K-N) and controls (G-J) showing downregulation of Fgf8 expression (arrows in K,G), Hoxd13 (H,L), Hoxa13 (I,M) and Wnt5a (J,N).