Fig. 4. Ncd concentration dependent gliding. The individual points represent the speeds of individual microtubule trajectories for different concentrations of Ncd (10 μg/ml about7.5 nM). The line in the graph is a guide to the eye to show the linear concentration dependence on gliding speed, up to a saturation point and maximum gliding speed of about 120 nm/s.

Fig. 5. A typical fluorescence time-series of Ncd gliding (12 nM) microtubules. The microtubules are fluorescently labeled (colors inverted) and are continuously driven unidirectionally. Arrows indicate the direction of microtubule gliding.

Movie 1. Membrane tubes formed by nonprocessive motors. Fluorescence movie of a membrane tube network extracted from a GUV by nonprocessive motors walking on microtubules on the underlying surface. The tubes in the lower left of the network exhibit the unique bidirectional behavior we report in our paper. The movie is accelerated 3× (scale bar ,10 μm).