Antioxidant-induced modification of INrf2 cysteine 151 and PKC-{delta}-mediated phosphorylation of Nrf2 serine 40 are both required for stabilization and nuclear translocation of Nrf2 and increased drug resistance -- Niture et al. 122 (24): 4452 Data Supplement - JCS058537 Supplementary Material -- Journal of Cell Science

JCS058537 Supplementary Material

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Supplemental Figure S1 -
Fig. S1. Hepa-1 cells transfected with FLAG-Nrf2 or mutant Nrf2S40A-GFP were treated with either DMSO or tBHQ for 2 hours and cell lysates immunoprecipitated with control IgG or anti-FLAG or anti-GFP or anti-Nrf2 pS40 antibody and immunoblotted with anti-FLAG, anti-GFP and anti-pSer40-Nrf2 antibody.