Fig. S1. Ablation of haemocytes in adult flies. (A) pxn- GAL4, UAS-GFP; TM6B/MKRS. (B) pxn-GAL4, UAS-GFP; TM6B/UAS-ice. Images were taken at 20× magnification. Sessile haemocytes were observed with both lines in the thorax and legs, as indicated by white arrowheads. Infection with either Enterococcus feacalis (Gram-positive bacterium; C) or E. coli (D) resulted in a reduced survival rate for the haemocyte deficient flies. These experiments were repeated five times independently and shown are mean values with ± standard deviation.

Fig. S2. Our study on the effect of haemocyte ablation on AMP gene induction following septic injury with E. coli was extended to defencin (def). Expression of def was measured by quantitative real-time PCR in whole larvae. Values shown are mean values (± standard deviation) from three independent experiments. Our results correlate with those of Brennan et al., 2007.

Fig. S3. Effects of haemocyte deficiency in AMP induction and survival following infection. (A) Survival of haemocyte-deficient larvae following E coli challenge. Larvae mutant for domino were very sensitive to infection with a 50% death rate at 4 hours after challenge. By contrast, hmlGAL4,UAS-GFP; UAS-ice survived as wild type larvae during the observation period of 6 hours. Similar results were observed with M. luteus (not shown). (B) At 3 hours post-infection with.E coli (dipt) or M luteus (drs), dom larvae had a severely reduced AMP response in comparison to wild-type larvae. The graph represents the mean values ± standard deviation of three independent experiments. Northerns were performed in whole larvae.