Transcriptomic Response of Listeria monocytogenes to Iron Limitation and fur Mutation
Appl. Environ. Microbiol. Ledala et al. 76: 406

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Legends to supplementary figures.
    MS Word document, 30K.
  • Supplemental file 2 - Growth of L. monocytogenes strains (Fig. S1); electrophoretic mobility shift assay to determine the interaction of purified Fur protein with radiolabeled promoter sequences (Fig. S2); Northern blot analyses to determine the expression of fur and feoB genes (Fig. S3) and of lmo0514 and lmo2185 (Fig. S4) from L. monocytogenes and to determine the expression of L. monocytogenes fur and lmo2185 in response to 2,2′-dipyridyl (Fig. S5).
    MS PowerPoint file, 675K.
  • Supplemental file 3 - Comparative gene expression in response to iron and fur mutation in L. monocytogenes (Table S1); identification of putative Fur binding sequences (Fur boxes) (Table S2); qRT-PCR analysis of the effects of ferric citrate and DPD on fur and FRC genes (Table S3); primers used for EMSA (Table S4).
    MS Word document, 1.04 MB.