Blood -- Molecular signatures to improve diagnosis in peripheral T-cell lymphoma and prognostication in angioimmunoblastic T-cell lymphoma

Blood, Vol. 115, Issue 5, 1026-1036, February 4, 2010

Molecular signatures to improve diagnosis in peripheral T-cell lymphoma and prognostication in angioimmunoblastic T-cell lymphoma
Blood Iqbal et al. 115: 1026

Supplemental materials for: Iqbal et al

Files in this Data Supplement:

Figure S1. Survival of PTCL patients included in this study (PDF, 144 KB) -
(A–B) Overall survival (OS) and event-free survival (EFS) of patients by pathologic diagnosis. ALK (+) ALCL had the best 5-year OS (>70%) and ATLL had the worst OS (Ki67 mRNA expression level.
Figure S2. Identification of consensus clusters (PDF, 84 KB) -
Top panel shows the consensus clusters in each of the algorithms. SOM (self-organizing map), KM (K-Means), HC (hierarchical clustering). Lower panel is a schematic diagram showing how the clusters are related in the different clustering algorithms using SOM clusters as the reference- SOM 1 and 2 correspond to HC 3, SOM 3 corresponds to HC 1, SOM 4 and 5 correspond to HC 2; SOM 1 to KM 1, SOM 2, 3 and 4 to KM2, and SOM 5 to KM 3. SOM 5 contains mainly normal lymphoid cells and cell lines. Excluding this cluster leaves four clusters in SOM. Based on the four clusters scheme, the following table shows where the major core clusters are located.
Figure S3 (PDF, 156 KB) -
(A) Distribution of molecularly classified AITL and PTCL-U according to the mean expression of a curated set of NF-κB pathway gene signature genes (MAP3K14, NFKBIA, NFKB2, RELA, TNFAIP3, CFLAR, MMP9, INFG, NFKBIE, and NFKB1). The average expression of these 10 most representative NF-κB pathway genes for T cells indicate that cases with high NF-κB are significantly associated with the AITL subgroup (p
Figure S4 (PDF, 135 KB) -
(A) An ALK(+) signature derived from cell line experiments shows marginal enrichment in ALK(+)ALCL patient samples, but does not reach statistical significance (B) Comparative analysis of ALK+ALCL classifier genes in cases diagnosed both molecularly and pathologically, molecularly only, and pathologically only.
Figure S5. Molecular characteristic of ALK (−) ALCL (PDF, 224 KB) -
Differential gene expression (A) ALK (−) ALCL vs PTCL-U (B) ALK (−) vs ALK(+) ALCL (C) Enrichment of STAT3 induced target gene signature in ALK(+)ALCL compared with ALK(−)ALCL and PTCL-U.

Supplemental tables can be found here.