Novel Antibiotic-Free Plasmid Selection System Based on Complementation of Host Auxotrophy in the NAD De Novo Synthesis Pathway -- Dong et al. 76 (7): 2295 Data Supplement - Supplemental material -- Applied and Environmental Microbiology

Novel Antibiotic-Free Plasmid Selection System Based on Complementation of Host Auxotrophy in the NAD De Novo Synthesis Pathway
Appl. Environ. Microbiol. Dong et al. 76: 2295

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Multiple sequence alignment of the deduced amino acid sequence of QAPRTase in different species (Fig. S1); primers and their designated application (Table S1); de novo pathways of NAD synthesis in different species (Table S2); percent sequence identity of QAPRTase in different species (Table S3); copy number of different plasmids in E. coli strain BW25113ΔQAPRTase (Table S4); also shown, in the last portion of the supplemental material, are the QAPRTase gene or protein sequences of three species (X. tropicalis, a lizard, and an opossum).
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