Kinetic Characterization of the WalRKSpn (VicRK) Two-Component System of Streptococcus pneumoniae: Dependence of WalKSpn (VicK) Phosphatase Activity on Its PAS Domain -- Gutu et al. 192 (9): 2346 Data Supplement - Supplemental material -- The Journal of Bacteriology

Kinetic Characterization of the WalRK_Spn (VicRK) Two-Component System of Streptococcus pneumoniae: Dependence of WalK_Spn (VicK) Phosphatase Activity on Its PAS Domain
J. Bacteriol. Gutu et al. 192: 2346

Supplemental material

Files in this Data Supplement:

Supplemental file 1 - Table S1, strains; Table S2, plasmids; Table S3, primers; Table S4, overexpression conditions, solubility, and concentration of proteins; Table S5, secondary structure of WalK_Spn constructs; Table S6, amino acids of the WalR_Spn response regulator expected to modulate autophosphatase rate; Fig. S1, SDS-PAGE of purified proteins; Fig. S2, insoluble constructs of WalK_Spn; Fig. S3 to S5, quantitation of autophosphorylation of WalK_SpnΔ35, WalK_SpnΔ195, and WalK_Spn PAS domain mutant constructs
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