Lipid phosphate phosphatase activity regulates dispersal and bilateral sorting of embryonic germ cells in Drosophila

DEV046110 Supplementary Material

Files in this Data Supplement:

  • Supplemental Figure S1 -

    Fig. S1. wun and wun2 double-mutant allele. (A) Genomic locus of wun and wun2 on chromosome 2R showing P-element excision-induced deletion of wun2 (wun2EP2650ex34) and the EMS-induced allele of wun (wun49). (B) Alignment of the protein sequence of human and fly lipid phosphate phosphatases (LPPs) with the site of the EMS-induced allele of wun indicated with an arrow.

  • Supplemental Table S1 -
  • Movie 1 -

    Movie 1. Wild-type germ cell dispersal. Maximum intensity projections of a lateral view of a stage-10 wild-type embryo. Germ cells, labelled using a nos>moeGFP construct, initially in a cluster in the posterior midgut pocket, disperse at the onset of transepithelial migration.

  • Movie 2 -

    Movie 2. Germ cell movement in a wun wun2 M−Z− embryo. Maximum intensity projections of a lateral view of a stage-11 wun wun2 M−Z− embryo. Germ cells have not dispersed at stage 10 and remain in a cluster in the posterior midgut. However, a few germ cells do break away from the cluster and move with amoeboid motion.