Blood -- Tissue type plasminogen activator regulates myeloid-cell dependent neoangiogenesis during tissue regeneration

Blood, Vol. 115, Issue 21, 4302-4312, May 27, 2010

Tissue type plasminogen activator regulates myeloid-cell dependent neoangiogenesis during tissue regeneration
Blood Ohki et al. 115: 4302

Supplemental materials for: Ohki et al

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Table S1. Coexpression of angiogenesis-related antigens on CD11b⁺ cells (PDF, 161 KB)
Table S2. Increased number of circulating CD11b⁺ and VEGFR-1⁺ cells in tPA-treated mice (PDF, 132 KB)
Figure S1. tPA promotes expression of angiogenesis- and cell migration-related genes in myeloid cells. CD11b⁺ CD11b⁻ (JPG, 272 KB) -
C57BL/6 mice were injected intraperitoneally (i.p.) with recombinant tPA from day 0 until day 2. (A) Peripheral blood mononuclear cells (PBMCs), isolated from tPA-treated or control C57BL/6 mice, were stained with CD45, CD11b, or F4/80 antibodies and analyzed by FACS on day 2. Flow cytometric analysis of the CD45⁺ cell population demonstrated the presence of the indicated CD11b and F4/80 subpopulations: CD11b^high, CD11b^med/F4/80^med, CD11b^low/F4/80⁻, and CD11b⁻/F4/80⁻(n=3). (B) RT-PCR analysis of VEGF-A,CXCR-4, CCR2, GM-CSF, neuropilin-1, and Tie-2 expression in CD11b⁺ and CD11b⁻ cells isolated from mice treated with tPA or BSA-PBS in vivo. β-actin served as an internal control (n=2).