Fig. S1. Encounter order does not affect residency time durations. Each panel shows the distribution of normalized residency durations for a cone type as shown in Fig. 6B, but segregated by the cone visited prior to the current cone (each row of a given panel indicates a different prior cone). Color code as shown in Fig. 1F. Axes and row labels are shown in A and apply to all panels. We used a Kruskal−Wallis test to test for differences in the distribution of within each panel and found none at P<0.01. (A) χ² 0.649, d.f.=3, N=190; (B) χ² 8.639, d.f.=3, N=192; and (C) χ² 2.846, d.f.=3, N=125.

Fig. S2. Basic statistics of locomotor behavior in flies with antennal immobilization. The two leftmost box plots in each panel are data for flies exploring an empty area in the light (white, N=34) and in the dark (gray, N=34). The two rightmost box plots are data from flies exploring the floor of the area with cones present in the light (white, N=40) and in darkness (gray, N=34). (A) The total distance traveled by individual flies during a 10-min trial. (B) The mean speed while the flies were walking. (C) The maximum speed while the flies were walking. (D) The percentage of the time the flies spent walking, normalized for the total time spent on the floor of the area when cones were present. (E) The mean angular speed while the flies were walking. Statistical comparisons were made using heteroscedastic two-sample t-tests unless the data were not normally distributed in which case the Mann−Whitney U-test was used. *Statistically different distributions (P<0.05 with Bonferroni correction).

Movie 1. Example of a fly exploring the arena with equal area cones present. A hungry, wing-clipped fly explores a large, free-walking arena with four cones of equal lateral surface area present. The footprint of the cones are outlined according to color code in Fig. 1F. The left side shows a reconstruction of the fly’s trajectory within the arena; the x−y position in the 50 previous frames are shown in white, beyond 50 previous frames the x−y positions are shown in gray. The right side shows the saved small-format images of the fly with tracking data (x−y position and body orientation) superimposed in red. This movie was recorded at 20 frames s⁻¹ in Arena 2 and plays back at ∼30 frames s⁻¹.

Movie 2. Example of a fly with immobilized antennae exploring the arena with equal area cones present in complete darkness. A hungry, wing-clipped fly with immobilized antennae explores a large, free-walking arena in complete darkness with four cones of equal lateral surface area present. The footprint of the cones are outlined according to color code in Fig. 1F. The left side shows a reconstruction of the fly’s trajectory within the arena; the x−y position in the 50 previous frames are shown in white, beyond 50 previous frames the x−y positions are shown in gray. The right side shows the saved small-format images of the fly with tracking data (x−y position and body orientation) superimposed in red. This movie was recorded at 20 frames s⁻¹ in Arena 2 and plays back at ∼30 frames s⁻¹.