Blood, Vol. 114, Issue 19, 4243-4252, November 5, 2009

Plasmodium falciparum–infected erythrocytes induce NF-B regulated inflammatory pathways in human cerebral endothelium
Blood Tripathi et al. 114: 4243

Supplemental materials for: Tripathi et al

Files in this Data Supplement:

• Table S1. Upregulated genes in secondary statistical analysis. p
  
• Table S2. Downregulated genes in secondary statistical analysis. p
  
• Figure S1.Scanning EM of the Pf-IRBC surface showing presence of knobs (JPG, 776 KB) -
  The scale bar is 1 micron and a single uninfected erythrocyte is shown in the center. Methods:Percoll purified unselected 3D7 were washed in PBS and spun gently on to polylysine 5 mm silica chips(Ted Pella, cat #16008). Chips were then stained with 2% uranyl acetate for 30–60 min. After one quick rinse with 50% ethanol, chips were rinsed consecutively in 50, 70, and 90% ethanol for 3 min each, followed by three rinses in 100% ethanol for 3 min each. After critical point drying for 1–2 h, chips were coated with chromium. Silica chips loaded with samples were ready for FEISEM (LEO 1550).
  
  
  
  
  
  
• Figure S2. Global distribution of raw fluorescence intensity values (log scale) of genes, across the four experimental conditions (JPG, 281 KB) -
  Raw expression values were normalized against medium control and distribution curve was generated in Gene Spring 6.1.1.