Supplementary material for Mazars et al. (2001) Proc. Natl. Acad. Sci. USA 98 (4), 1901–1906. (10.1073/pnas.040339798)

Table 4.

 Conditions for PCR amplification of 12 Mycobacterium tuberculosis mycobacterial interspersed repetitive unit-variable number tandem repeat loci

Locus

PCR primers (5' to 3' )

MgCl2 concentration, mM

MIRU 2

TGGACTTGCAGCAATGGACCAACT

TACTCGGACGCCGGCTCAAAAT

2

MIRU 4

GCGCGAGAGCCCGAACTGC

GCGCAGCAGAAACGTCAGC

2

MIRU 10

GTTCTTGACCAACTGCAGTCGTCC

GCCACCTTGGTGATCAGCTACCT

2

MIRU 16

TCGGTGATCGGGTCCAGTCCAAGTA

CCCGTCGTGCAGCCCTGGTAC

2

MIRU 20

TCGGAGAGATGCCCTTCGAGTTAG

GGAGACCGCGACCAGGTACTTGTA

1.5

MIRU 23

CAGCGAAACGAACTGTGCTATCAC

CGTGTCCGAGCAGAAAAGGGTAT

2.5

MIRU 24

CGACCAAGATGTGCAGGAATACAT

GGGCGAGTTGAGCTCACAGAA

1.5

MIRU 26

CCCGCCTTCGAAACGTCGCT

TGGACATAGGCGACCAGGCGAATA

1

MIRU 27

TCGAAAGCCTCTGCGTGCCAGTAA

GCGATGTGAGCGTGCCACTCAA

1.5

MIRU 31

ACTGATTGGCTTCATACGGCTTTA

GTGCCGACGTGGTCTTGAT

2

MIRU 39

CGCATCGACAAACTGGAGCCAAAC

CGGAAACGTCTACGCCCCACACAT

2.5

MIRU 40

GGGTTGCTGGATGACAACGTGT

GGGTGATCTCGGCGAAATCAGATA

2