New Method for Counting Bacteria Associated with Coral Mucus
Appl. Environ. Microbiol. Garren and Azam 76: 6128
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Method for additional trypsinization optimization experiments.
MS Word document, 27K. - Supplemental file 2 - Legends for supplemental figures.
MS Word document, 25K. - Supplemental file 3 - Trials to reduce autofluorescence of mucus samples prior to DAPI staining (Fig. S1).
Zipped TIF Image, 101K. - Supplemental file 4 - Average bacterial counts from mucus samples treated with EDTA/trypsin alone or with crystal violet, acidic alcian blue, or FeSO4 to quench autofluorescence before DAPI staining, and abundances of zooxanthellae from the same samples (Fig. S2).
Zipped TIF Image, 101K. - Supplemental file 5 - The number of bacteria enumerated in A. youngii mucus (with and without trypsinization) and the water carried along during mucus sampling using two separate collection methods (Fig. S3).
Zipped TIF Image, 76K. - Supplemental file 6 - Results of incubation of mucus samples with 0.2 or 0.4% trypsin at room temperature or 37°C for different time periods (Fig. S4).
Zipped TIF Image, 104K.