Ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene Pto -- Data Supplement - Supplemental Figures -- Proceedings of the National Academy of Sciences

Supplementary material for Riely and Martin (2001) Proc. Natl. Acad. Sci. USA 98 (4), 2059–2064. (10.1073/pnas.031480498)

Fig. 6.
Agrobacterium-mediated transient expression assay of the LhirPto genes. LhirPto genes were placed under control of the cauliflower mosaic virus (CaMV) 35S promoter and cloned into the pBTEX plasmid, which also contained a CaMV 35S::avrPto gene. The constructs were transformed into Agrobacterium tumefaciens strain EHA105. A. tumefaciens strains were induced with acetosyringone and syringe-infiltrated into mature Nicotiana benthamiana leaves. Photographs were taken 4 days after infiltration. The constructs infiltrated in the indicated panels are as follows: A, 35S::Pto + 35S::avrPto; B, 35S::LhirPto; C, 35S::avrPto; D, 35S::LhirPto + 35S::avrPto; E, 35S::Lhirfen + 35S::avrPto; F, 35S::LhirPtoD + 35S::avrPto; and G, 35S::LhirPtoF + 35S::avrPto.