Blood -- B7-H1/CD80 interaction is required for the induction and maintenance of peripheral T-cell tolerance

Blood, Vol. 116, Issue 8, 1291-1298, August 26, 2010

B7-H1/CD80 interaction is required for the induction and maintenance of peripheral T-cell tolerance
Blood Park et al. 116: 1291

Supplemental materials for: Park et al

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Figure S1. Expression of B7-H1 on cell surface after 43H12 binding (PDF, 54.7 KB) -
B7-H1-positive P815 cells were incubated in the presence of 10 µg/ml 43H12 (bottom panels) or control rat IgG (middle panels) for 1 hr at 37°C. The cells were washed and incubated for additional 1 day (left column) or 3 days (right column), and expression level of B7-H1 was examined by a staining with 10B4 mAb, which recognizes B7-H1 epitope distinct from 43H12. As a control, 10B4 staining of B7-H1-positive P815 without mAb incubation is also shown (top panel). Filled histogram indicates staining with 10B4, while open histogram shows control staining with secondary Ab alone. Representative data from 2 independently repeated experiments are shown.
Figure S2. In vivo effects of 43H12 in the presence or absence of CD80-CD28/CTLA-4 interaction (PDF, 52.1 KB) -
B6 mice were transferred i.v. with OT-I T cells and injected i.v. with 0.5 mg OVA peptide on day 0. The mice were treated i.p. with or without anti-CD80 mAb 16-10A1 (200 µg/mouse) on day 0. On day 0 and 3, the mice were injected i.p. with 43H12 or control Ig (200 µg/mouse). On day 5, splenocytes were harvested from the mice, and the percentage of OT-I T cells in CD8-positive population was assessed by flow cytometry. Representative data from 2 independently repeated experiments are shown.
Figure S3. IEL compartment after 43H12 treatment in mice with oral tolerance (PDF, 76 KB) -
B6 mice were given drinking water supplemented with OVA protein from day 0 to 7. The mice were immunized s.c. with OVA protein emulsified in CFA on day 14, and treated i.p. with 150 µg 43H12 (right panels) or control rat IgG (left panels) on day 0, 4, 8, and 12. On day 21, IEL were harvested and analyzed by a flow cytometry. Percentages of CD8aa T cells (upper panels) and gdT cells (lower panels) in whole IEL are indicated. Representative data from 2 independently repeated experiments are shown.