Blood -- Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis

Blood, Vol. 113, Issue 15, 3461-3471, April 9, 2009

Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis
Blood Gekas et al. 113: 3461

Supplemental materials for: Gekas et al

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Table S1. Complete blood cytometry of Mef2C decient mice (PDF, 56.1 KB) -
Complete blood cytometry (CBC) data for blood parameters of young (1–3 months) and older (6–12 months) VavCre⁺Mef2C^fl∕fl and age-matched littermate control mice. The table is grouped for platelet, erythroid and leykocyte compartments. PLT; platelet count, MPV; mean platelet volume, PDW; platelet distribution width, RBC; red blood cell count, MCV; mean corpuscular volume, HB, hemoglobin, HCT; hematocrit, MCH; mean corpuscular hemoglobin, MCHC; mean corpuscular hemoglobin concentration, RDW; red cell distribution width, WBC, white blood cell count, NE; neutrophil, LY; lymphoid, MO; monocyte, EO; eosinophil, BA; basophil.
Figure S1. FACS analysis of hematopoietic lineages in peripheral blood and spleen (JPG, 159 KB) -
(A–C) Representative plots show frequencies of Gr1/Mac1 myeloid cells, CD4/CD8 T cells and B220⁺IgM⁺ B cells in young (1–3 month; mo) and older (6–12) mice. Gated populations are Gr1⁺Mac1⁺ granulocytes, Mac1⁺ monocytes, CD4⁺ T-helper cells and CD8a⁺ cytotoxic T cells. (A) A slight increase in Gr1⁺Mac1⁺ granulocytes was observed in older but not in young VavCre⁺Mef2C^fl∕fl mice. (B) T-lymphoid development is not impaired in the absence of Mef2C. (C) FACS analysis of whole blood showed a slight reduction in the frequency of circulating B220⁺IgM⁺ B cells, in both young and older VavCre⁺Mef2C^fl∕fl mice. (Young mice: controls, N=8; VavCre⁺Mef2C^fl∕fl, N=3. Older mice: controls, N=7; VavCre⁺Mef2C^fl∕fl, N=7). (D) Spleens from VavCre⁺Mef2C^fl∕fl mice were analyzed for presence of B220⁺IgM⁺ B cells. Despite loss of B-cell subsets in bone marrow seen in older mice, spleen B-lymphopoiesis occurs at comparable levels to age-matched littermates (N=3). Values represent mean frequencies ± SD.