Limitations in Xylose-Fermenting Saccharomyces cerevisiae, Made Evident through Comprehensive Metabolite Profiling and Thermodynamic Analysis
Appl. Environ. Microbiol. Klimacek et al. 76: 7566

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Short description of the preparation of xylulose-5-phosphate, overview of all strains used (Table S1), all compounds analyzed by LC-MS/MS together with the respective masses for identification (Table S2), quantitative summary of measured metabolite pools (Table S3), results of thermodynamic analysis (Table S4), summaries of metabolic control analysis of reactions constituting the xylose assimilation pathway (Table S5) and catalyzed by PFK, PYK, TAL, and TKL (Table S6), pathways for D-xylose and L-arabinose assimilation (Fig. S1), typical elution profiles of pentose-5-phosphate isomers (Fig. S2), and differences in metabolite levels of xylose-metabolizing and xylose-resting cells relative to glucose-growing cells (heat maps) (Fig. S3).
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