The ADP-Ribosylation Domain of Pseudomonas aeruginosa ExoS Is Required for Membrane Bleb Niche Formation and Bacterial Survival within Epithelial Cells
Infect. Immun. Angus et al. 78: 4500

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 - Movie S1. Wild-type P. aeruginosa strain PAO1 localized to the cytosol of human corneal epithelial cells at 8 h postinfection.
    MPEG-4 Movie file, 414K.
  • Supplemental file 2 - Movie S1-1. Wild-type P. aeruginosa strain PAO1 localized to a mature membrane bleb induced in human corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 1.7 MB.
  • Supplemental file 3 - Movie S2. P. aeruginosa PAO1 ΔexoS trapped inside intracellular vacuoles in human corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 1 MB.
  • Supplemental file 4 - Movie S2-1. P. aeruginosa PAO1 exsA::Ω trapped inside numerous intracellular vacuoles in rabbit corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 12.5 MB.
  • Supplemental file 5 - Movie S3. Mature membrane bleb containing P. aeruginosa PAO1 ΔexoT ΔexoY in human corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 732K.
  • Supplemental file 6 - Movie S4. Mature membrane bleb containing P. aeruginosa PAO1 ΔexoS ΔexoT ΔexoY pUCP18_exoS in human corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 1 MB.
  • Supplemental file 7 - Movie S5. P. aeruginosa PAO1 ΔexoS ΔexoT ΔexoY pUCP18_exoS_E381D trapped inside intracellular vacuoles in rabbit corneal epithelial cells 8 h postinfection.
    QuickTime Movie file, 446K.
  • Supplemental file 8 - Movie S6. Membrane bleb containing P. aeruginosa PAO1 ΔexoS ΔexoT ΔexoY pUCP18_exoS_R146K within human corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 3.9 MB.
  • Supplemental file 9 - Movie S7. P. aeruginosa PAO1 ΔexoS ΔexoT ΔexoY pUCP18_exoSΔ51-77 is also localized to mature membrane blebs in human corneal epithelial cells at 8 h postinfection.
    QuickTime Movie file, 1.35 MB.
  • Supplemental file 10 - Legends for Movies S1 through S7.
    MS Word document, 67K.