K+ limitation | kdpF, kdpA, kdpB, kdpC | induction | 95158698 | |||
Osmotic Shock | betT | betT | induction | 96198169 | ||
Low pH | CadC | cadB | cadB, cadA | induction | 96404804, 94252996 | |
Anaerobiosis | phosphate group | ArcA | betI | betI, betB, betA | repression | 96198169 |
Sulfate Starvation | N-acetylserine | CysB | tauA | tauA, tauB, tauC, tauD | induction | 98062196 |
Glucose Starvation | cAMP | CRP | malT | malT | induction | 95379487 |
This table contains some few examples that illustrate the type of information that is encoded in RegulonDB concerning conditions of growth of E. coli whose effect -either inducing or repressing- some genes, is known. What we want to emphasize is that the database can encode information on systems where the molecular biology is known in detail, as well as systems where only genetic information is available.
To deal with cases where the molecular biology is known, this table includes promoters, proteins, and effector molecules directly involved in the expression of the genes under specific conditions. Thus, for instance, only under sulfate starvation, N-acetylserine binds to CysB, a transcriptional regulator that will directly active the expression of the genes tauABCD, via the tauA promoter (See 98062196).
If a regulatory protein affects the expression of a gene but a direct relationship has not been demonstrated with the condition, then we only included the condition and the affected genes. This is the example of induction of genes under potassium limiation (See 95158698).
A case with intermediate knowledge, is that of the induction of the cadB and cadA genes by the CadC transcriptional regulator at low pH. However, the precise effector molecule that binds to CadC at low pH is still unknown (See 6404804, 94252996).
As can be deduced, the only required information is that of the condition, the effect of induction or repression and the set of affected genes. We are on the other hand aware, that this encoding is still subject to enrichment to deal with representing elaborate pathways of signal transduction.