<?xml version="1.0"?>
<!DOCTYPE BIND-Interaction SYSTEM "ncbi.dtd">
<BIND-Interaction>
<BIND-Interaction_date>
<Date>
<Date_std>
<Date-std>
<Date-std_year>1999</Date-std_year>
<Date-std_month>8</Date-std_month>
<Date-std_day>18</Date-std_day>
</Date-std>
</Date_std>
</Date>
</BIND-Interaction_date>
<BIND-Interaction_iid>
<Interaction-id>10</Interaction-id>
</BIND-Interaction_iid>
<BIND-Interaction_a>
<BIND-object>
<BIND-object_short-label>LAT</BIND-object_short-label>
<BIND-object_id>
<BIND-object-type-id>
<BIND-object-type-id_protein>
<BIND-id>
<BIND-id_gi>
<Geninfo-id>2828026</Geninfo-id>
</BIND-id_gi>
<BIND-id_di>
<Domain-id>0</Domain-id>
</BIND-id_di>
</BIND-id>
</BIND-object-type-id_protein>
</BIND-object-type-id>
</BIND-object_id>
<BIND-object_origin>
<BIND-object-origin>
<BIND-object-origin_org>
<BioSource>
<BioSource_org>
<Org-ref>
<Org-ref_taxname>Homo sapiens</Org-ref_taxname>
<Org-ref_db>
<Dbtag>
<Dbtag_db>taxon</Dbtag_db>
<Dbtag_tag>
<Object-id>
<Object-id_id>9606</Object-id_id>
</Object-id>
</Dbtag_tag>
</Dbtag>
</Org-ref_db>
</Org-ref>
</BioSource_org>
</BioSource>
</BIND-object-origin_org>
</BIND-object-origin>
</BIND-object_origin>
<BIND-object_descr>Linker for Activation of T cells; contains amino-terminal transmembrane domain</BIND-object_descr>
</BIND-object>
</BIND-Interaction_a>
<BIND-Interaction_b>
<BIND-object>
<BIND-object_short-label>PLC-gamma</BIND-object_short-label>
<BIND-object_id>
<BIND-object-type-id>
<BIND-object-type-id_protein>
<BIND-id>
<BIND-id_gi>
<Geninfo-id>130225</Geninfo-id>
</BIND-id_gi>
<BIND-id_di>
<Domain-id>0</Domain-id>
</BIND-id_di>
</BIND-id>
</BIND-object-type-id_protein>
</BIND-object-type-id>
</BIND-object_id>
<BIND-object_origin>
<BIND-object-origin>
<BIND-object-origin_org>
<BioSource>
<BioSource_org>
<Org-ref>
<Org-ref_taxname>Homo sapiens</Org-ref_taxname>
<Org-ref_db>
<Dbtag>
<Dbtag_db>taxon</Dbtag_db>
<Dbtag_tag>
<Object-id>
<Object-id_id>9606</Object-id_id>
</Object-id>
</Dbtag_tag>
</Dbtag>
</Org-ref_db>
</Org-ref>
</BioSource_org>
</BioSource>
</BIND-object-origin_org>
</BIND-object-origin>
</BIND-object_origin>
<BIND-object_descr>phospholipase C; contains one C2, two PH, two SH2, one SH3 and one EF-hand calcium binding domains.</BIND-object_descr>
</BIND-object>
</BIND-Interaction_b>
<BIND-Interaction_descr>
<BIND-descr>
<BIND-descr_simple-descr>Phospholipase C-gamma1 interacts with membrane bound LAT.</BIND-descr_simple-descr>
<BIND-descr_place>
<BIND-place-set>
<BIND-place-set_max-bpid>
<BIND-place-id>0</BIND-place-id>
</BIND-place-set_max-bpid>
<BIND-place-set_places>
<BIND-place>
<BIND-place_bpid>
<BIND-place-id>0</BIND-place-id>
</BIND-place_bpid>
<BIND-place_gen-place>
<BIND-gen-place-set>
<BIND-gen-place-set_start>
<BIND-gen-place>
<BIND-gen-place_cytoplasmic-membrane>
<BIND-membrane value="inner-surface"/>
</BIND-gen-place_cytoplasmic-membrane>
</BIND-gen-place>
</BIND-gen-place-set_start>
</BIND-gen-place-set>
</BIND-place_gen-place>
<BIND-place_source>
<BIND-pub-set>
<BIND-pub-set_disputed value="false"/>
<BIND-pub-set_pubs>
<BIND-pub-object>
<BIND-pub-object_descr>LAT: The ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>9489702</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
<BIND-pub-object>
<BIND-pub-object_descr>Grb2 and phospholipase C-gamma 1 associate with a 36- to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>7516467</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
</BIND-pub-set_pubs>
</BIND-pub-set>
</BIND-place_source>
<BIND-place_descr>LAT is a membrane bound protein. The LAT-PLCgamma1 interaction is found in the particulate fraction of fractionated cells.</BIND-place_descr>
</BIND-place>
</BIND-place-set_places>
</BIND-place-set>
</BIND-descr_place>
<BIND-descr_cond>
<BIND-condition-set>
<BIND-condition-set_max-icid>
<Internal-conditions-id>3</Internal-conditions-id>
</BIND-condition-set_max-icid>
<BIND-condition-set_conditions>
<BIND-condition>
<BIND-condition_icid>
<Internal-conditions-id>0</Internal-conditions-id>
</BIND-condition_icid>
<BIND-condition_general value="in-vitro"/>
<BIND-condition_system>
<BIND-experimental-system value="immunoprecipitation">18</BIND-experimental-system>
</BIND-condition_system>
<BIND-condition_descr>TCR stimulated Jurkat T cell lysates were immunoprecipitated with anti-PLCgamma1 and immunoblotted with anti-phosphotyrosine antibodies. Fig. 2A.</BIND-condition_descr>
<BIND-condition_source>
<BIND-pub-set>
<BIND-pub-set_disputed value="false"/>
<BIND-pub-set_pubs>
<BIND-pub-object>
<BIND-pub-object_descr>Lymphocyte lineage-restricted tyrosine-phosphorylated proteins that bind PLC gamma 1 SH2 domains.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>1320023</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
</BIND-pub-set_pubs>
</BIND-pub-set>
</BIND-condition_source>
</BIND-condition>
<BIND-condition>
<BIND-condition_icid>
<Internal-conditions-id>1</Internal-conditions-id>
</BIND-condition_icid>
<BIND-condition_general value="in-vitro"/>
<BIND-condition_system>
<BIND-experimental-system value="affinity-chromatography">2</BIND-experimental-system>
</BIND-condition_system>
<BIND-condition_descr>TCR stimulated Jurkat T cell lysates incubated with PLCgamma1 SH2-Ig fusion protein followed by anti-phosphotyrosine immunoblotting. Fig. 2B.</BIND-condition_descr>
<BIND-condition_source>
<BIND-pub-set>
<BIND-pub-set_disputed value="false"/>
<BIND-pub-set_pubs>
<BIND-pub-object>
<BIND-pub-object_descr>Lymphocyte lineage-restricted tyrosine-phosphorylated proteins that bind PLC gamma 1 SH2 domains.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>1320023</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
</BIND-pub-set_pubs>
</BIND-pub-set>
</BIND-condition_source>
</BIND-condition>
<BIND-condition>
<BIND-condition_icid>
<Internal-conditions-id>2</Internal-conditions-id>
</BIND-condition_icid>
<BIND-condition_general value="in-vitro"/>
<BIND-condition_system>
<BIND-experimental-system value="other">255</BIND-experimental-system>
</BIND-condition_system>
<BIND-condition_descr>Far Western: TCR stimulated Jurkat T cell lysates were immunoprecipitated with anti-PLCgamma1 antibodies, separated by SDS-PAGE and transferred to immobilon. PLCgamma1 SH2-Ig was then incubated with the membrane. Fig. 2C.</BIND-condition_descr>
<BIND-condition_source>
<BIND-pub-set>
<BIND-pub-set_disputed value="false"/>
<BIND-pub-set_pubs>
<BIND-pub-object>
<BIND-pub-object_descr>Lymphocyte lineage-restricted tyrosine-phosphorylated proteins that bind PLC gamma 1 SH2 domains.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>1320023</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
</BIND-pub-set_pubs>
</BIND-pub-set>
</BIND-condition_source>
</BIND-condition>
<BIND-condition>
<BIND-condition_icid>
<Internal-conditions-id>3</Internal-conditions-id>
</BIND-condition_icid>
<BIND-condition_general value="in-vitro"/>
<BIND-condition_system>
<BIND-experimental-system value="immunoprecipitation">18</BIND-experimental-system>
</BIND-condition_system>
<BIND-condition_descr>TCR stimulated Jurkat T cell lysates were immunoprecipitated with anti-PLCgamma1 antibodies and immunoblotted with anti-LAT. Fig. 6B. The reverse IP-IB was also performed. Fig. 6C.</BIND-condition_descr>
<BIND-condition_source>
<BIND-pub-set>
<BIND-pub-set_disputed value="false"/>
<BIND-pub-set_pubs>
<BIND-pub-object>
<BIND-pub-object_descr>LAT: The ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>9489702</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
</BIND-pub-set_pubs>
</BIND-pub-set>
</BIND-condition_source>
</BIND-condition>
</BIND-condition-set_conditions>
</BIND-condition-set>
</BIND-descr_cond>
<BIND-descr_binding-sites>
<BIND-loc>
<BIND-loc_general>
<BIND-loc-gen>
<BIND-loc-gen_b-sites>
<BIND-loc-site-set>
<BIND-loc-site-set_max-slid>
<BIND-Seq-loc-id>0</BIND-Seq-loc-id>
</BIND-loc-site-set_max-slid>
<BIND-loc-site-set_sites>
<BIND-loc-site>
<BIND-loc-site_slid>
<BIND-Seq-loc-id>0</BIND-Seq-loc-id>
</BIND-loc-site_slid>
<BIND-loc-site_site>
<Seq-loc>
<Seq-loc_mix>
<Seq-loc-mix>
<Seq-loc>
<Seq-loc_feat>
<Feat-id>
<Feat-id_local>
<Object-id>
<Object-id_str>SH2 domain</Object-id_str>
</Object-id>
</Feat-id_local>
</Feat-id>
</Seq-loc_feat>
</Seq-loc>
</Seq-loc-mix>
</Seq-loc_mix>
</Seq-loc>
</BIND-loc-site_site>
</BIND-loc-site>
</BIND-loc-site-set_sites>
</BIND-loc-site-set>
</BIND-loc-gen_b-sites>
</BIND-loc-gen>
</BIND-loc_general>
</BIND-loc>
</BIND-descr_binding-sites>
<BIND-descr_intramolecular value="false"/>
</BIND-descr>
</BIND-Interaction_descr>
<BIND-Interaction_source>
<BIND-pub-set>
<BIND-pub-set_disputed value="false"/>
<BIND-pub-set_pubs>
<BIND-pub-object>
<BIND-pub-object_descr>Lymphocyte lineage-restricted tyrosine-phosphorylated proteins that bind PLC gamma 1 SH2 domains.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>1320023</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
<BIND-pub-object>
<BIND-pub-object_descr>Grb2 and phospholipase C-gamma 1 associate with a 36- to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>7516467</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
<BIND-pub-object>
<BIND-pub-object_descr>LAT: The ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.</BIND-pub-object_descr>
<BIND-pub-object_opinion value="support"/>
<BIND-pub-object_pub>
<Pub>
<Pub_muid>9489702</Pub_muid>
</Pub>
</BIND-pub-object_pub>
</BIND-pub-object>
</BIND-pub-set_pubs>
</BIND-pub-set>
</BIND-Interaction_source>
<BIND-Interaction_priv value="false"/>
</BIND-Interaction>