BIND-Interaction ::= {
  date
    std {
      year 1999 ,
      month 8 ,
      day 18 } ,
  iid 10 ,
  a {
    short-label "LAT" ,
    id
      protein {
        gi 2828026 ,
        di 0 } ,
    origin
      org {
        org {
          taxname "Homo sapiens" ,
          db {
            {
              db "taxon" ,
              tag
                id 9606 } } } } ,
    descr "Linker for Activation of T cells; contains amino-terminal
 transmembrane domain" } ,
  b {
    short-label "PLC-gamma" ,
    id
      protein {
        gi 130225 ,
        di 0 } ,
    origin
      org {
        org {
          taxname "Homo sapiens" ,
          db {
            {
              db "taxon" ,
              tag
                id 9606 } } } } ,
    descr "phospholipase C; contains one C2, two PH, two SH2, one SH3 and one
 EF-hand calcium binding domains." } ,
  descr {
    simple-descr "Phospholipase C-gamma1 interacts with membrane bound LAT." ,
    place {
      max-bpid 0 ,
      places {
        {
          bpid 0 ,
          gen-place {
            start
              cytoplasmic-membrane inner-surface } ,
          source {
            disputed FALSE ,
            pubs {
              {
                descr "LAT: The ZAP-70 tyrosine kinase substrate that links T
 cell receptor to cellular activation." ,
                opinion support ,
                pub
                  muid 9489702 } ,
              {
                descr "Grb2 and phospholipase C-gamma 1 associate with a 36-
 to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation." ,
                opinion support ,
                pub
                  muid 7516467 } } } ,
          descr "LAT is a membrane bound protein. The LAT-PLCgamma1
 interaction is found in the particulate fraction of fractionated cells." } } } ,
    cond {
      max-icid 3 ,
      conditions {
        {
          icid 0 ,
          general in-vitro ,
          system immunoprecipitation ,
          descr "TCR stimulated Jurkat T cell lysates were immunoprecipitated
 with anti-PLCgamma1 and immunoblotted with anti-phosphotyrosine antibodies.
 Fig. 2A." ,
          source {
            disputed FALSE ,
            pubs {
              {
                descr "Lymphocyte lineage-restricted tyrosine-phosphorylated
 proteins that bind PLC gamma 1 SH2 domains." ,
                opinion support ,
                pub
                  muid 1320023 } } } } ,
        {
          icid 1 ,
          general in-vitro ,
          system affinity-chromatography ,
          descr "TCR stimulated Jurkat T cell lysates incubated with PLCgamma1
 SH2-Ig fusion protein followed by anti-phosphotyrosine immunoblotting. Fig.
 2B." ,
          source {
            disputed FALSE ,
            pubs {
              {
                descr "Lymphocyte lineage-restricted tyrosine-phosphorylated
 proteins that bind PLC gamma 1 SH2 domains." ,
                opinion support ,
                pub
                  muid 1320023 } } } } ,
        {
          icid 2 ,
          general in-vitro ,
          system other ,
          descr "Far Western: TCR stimulated Jurkat T cell lysates were
 immunoprecipitated with anti-PLCgamma1 antibodies, separated by SDS-PAGE and
 transferred to immobilon. PLCgamma1 SH2-Ig was then incubated with the
 membrane. Fig. 2C." ,
          source {
            disputed FALSE ,
            pubs {
              {
                descr "Lymphocyte lineage-restricted tyrosine-phosphorylated
 proteins that bind PLC gamma 1 SH2 domains." ,
                opinion support ,
                pub
                  muid 1320023 } } } } ,
        {
          icid 3 ,
          general in-vitro ,
          system immunoprecipitation ,
          descr "TCR stimulated Jurkat T cell lysates were immunoprecipitated
 with anti-PLCgamma1 antibodies and immunoblotted with anti-LAT. Fig. 6B. The
 reverse IP-IB was also performed. Fig. 6C." ,
          source {
            disputed FALSE ,
            pubs {
              {
                descr "LAT: The ZAP-70 tyrosine kinase substrate that links T
 cell receptor to cellular activation." ,
                opinion support ,
                pub
                  muid 9489702 } } } } } } ,
    binding-sites {
      general {
        b-sites {
          max-slid 0 ,
          sites {
            {
              slid 0 ,
              site
                mix {
                  feat
                    local
                      str "SH2 domain" } } } } } } ,
    intramolecular FALSE } ,
  source {
    disputed FALSE ,
    pubs {
      {
        descr "Lymphocyte lineage-restricted tyrosine-phosphorylated proteins
 that bind PLC gamma 1 SH2 domains." ,
        opinion support ,
        pub
          muid 1320023 } ,
      {
        descr "Grb2 and phospholipase C-gamma 1 associate with a 36- to
 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation." ,
        opinion support ,
        pub
          muid 7516467 } ,
      {
        descr "LAT: The ZAP-70 tyrosine kinase substrate that links T cell
 receptor to cellular activation." ,
        opinion support ,
        pub
          muid 9489702 } } } ,
  priv FALSE }