Supporting information for Swaneye et al. (2003) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0237333100
Fig. 5.
Experimental validation of the linear detection range of the LumiImager. Detergent lysates of BaF3-EpoR cells were prepared, and the protein concentration contained was determined by Bradford assay. One to fifty mircograms of protein from total cell lysate was separated by 15% SDS/PAGE and analyzed by immunoblotting using an anti-STAT5 antiserum followed by chemiluminescence detection. After 10min exposure on the LumiImager, the immunoblot was quantified by using lumianalyst software, applying the single band analysis package with automated lane and band identification, flat background correction, and slant correction. Plotted are the protein concentration of total cell lysates versus the measured Boehringer Light Units (B.L.U.). Per protein concentration, the average of three measurements ± SD is depicted.